Study On Antioxidant Activities Of Oligopeptides From Fish Skin And Deer Blood

In this paper, the antioxidant activities of oligopeptides from fish skin and deer blood were evaluated by DPPH· assay,·OH assay, and PC12cells oxidatived model by MTT assay. In addition, the amino acid composition was determined to evaluate the nutritional value of oligopeptides from fish skin and deer blood. The results would provide some references for the application of oligopeptides from fish skin and deer blood. The main contents are as follows:1. Study on the scavenging free radicals capacities of oligopeptides from fish skin and deer blood. The capacities for scavenging free radicals of oligopeptides from fish skin and deer blood were evaluated by DPPH· assay and·OH assay, respectively. Under the optimum conditions using glutathione and ascorbic acid as the controls, the values of half-inhibitory concentration (IC50) of fish skin oligopeptides, deer blood oligopeptides, glutathione and ascorbic acid for OH were2.482,4.520,0.357and0.100mg/mL, respectively, and those for DPPH·were0.635,2.520,0.030and0.003mg/mL, respectively. The results showed that oligopeptides from fish skin and deer blood have potentially exhibited activities for free radicals.2. Study on the antioxidant activities of oligopeptides from fish skin and deer blood by PC12cells model. The protective effects of oligopeptides from fish skin and deer blood against PC12cells induced by H2O2were evaluated. The results have shown that the injury of PC12cells induced by H2O2could be reduced obviously by fish skin oligopeptide at high dose (P<0.05). While every dose group of the deer blood oligopeptides could reduce the injury of PC12cells (P<0.05).3. The nutritional value evaluation of amino acid of oligopeptides from fish skin and deer blood. The nutritional values of the amino acid from fish skin and deer blood oligopeptides were evaluated. According to the FAO/WHO model and whole egg protein model, amino acid score (AAS), chemical score (CS) and essential amino acids index (EAAI) are calculated, respectively. The results have shown that the amino acids content of two oligopeptides are higher than other daily foods and the essential amino acids are complete and rich. Tryptophan is the first limiting amino acid in these two oligopeptides. Except the tryptophan, the content of other essential amino acids are higher than the value recommended by WHO/FAO, which can fully meet the needs of the human body. Especially, the content of threonine, valine, leucine and lysine are high. In conclusion, the oligopeptides from fish skin and deer blood are the significant resources as functional food additives.