Warm Disease Clearing Lung Detoxification On Influenza Virus FM1Infected Mice Lung Cytokines IL-1Beta TNF Alpha IFN-Gamma Expression

Nowadays, modern medicine is still a lack of specificity against influenza virusdrugs, and traditional Chinese medicine in the treatment of viral upper respiratorytract infection and viral pneumonia has accumulated valuable theoretical and practicalknowledge.Clear lung detoxification is clear lung heat, dispel toxin factor,is the doctor oftraditional Chinese medicine science of seasonal febrile diseases for toxic heat indicatesthe lungs and the proposed solution treatment,Qing dynasty doctor Ye Tianshi lungfever, Chen Pingbo treatment wind temperature to make good use of the law.Allprevious dynasties to the formula of clear lung detoxification formula is numerous, thisresearch use of clearing lung detoxification, for the hospital (the first affiliated hospitalof anhui medical university) lung clinical experience.The clinical use and cure causedby influenza virus FM1viral upper respiratory tract infection and viral pneumoniaeffect is good, but for the benefit of the parties on influenza virus FM1effectmechanism is unclear.Guided by the theory of science of seasonal febrile diseases, this study by influenzavirus FM1infected mice experiments, explore clear lung detoxification mechanism ofaction of anti influenza virus FM1mice, reveals the scientific connotation of theparty.For clinical treatment of viral upper respiratory tract infection caused by theinfluenza virus FM1and provide experimental basis for viral pneumonia.This experiment will kunming mice were randomly divided into normal group,model group, western medicine group, the clear lung detoxification partygroup.Intranasal mice infected with influenza virus FM1strain after2hours, lavagetreatment group, normal group and model group give the same amount of saline lavage,infection after5days to be put to death.In the lung tissue of mice weighing, calculationlung and lung index in inhibiting rate; Lung biopsy, wood grain-eosin (HE) staining,and then observe the inflammatory lesions of lung tissue; By SABC immunohistochemical method to detect lung biopsy in cytokine IL-1beta, IFN-gamma, the expression of TNF alpha, analysis the meaning of each index change.In our experiment, we found:1, compared with model group, clearing lungdetoxification party were significantly decreased lung index of mice (P <0.01),suggesting the can effectively reduce the influenza virus FM1infected mice lung index,improve lung index inhibition rate;2, compared with model group, clearing lungdetoxification group significantly reduce the lung tissue pathology injury in mice, showthat the can effectively improve lung inflammatory lesions;3, compared with modelgroup, the clear lung detoxification party group in the lung tissue of mice IL-1beta,the expression of TNF alpha protein lowered obviously, IFN-gamma proteinexpression increases obviously,(P<0.01), suggesting that can inhibit theproinflammatory factor beta, IL-1TNF alpha and stimulate the production of IFN-gamma inflammation suppression factor.To sum up, this research confirmed that warm disease clearing lung detoxification inorder to improve the influenza virus FM1infected mice lung inflammation, has goodtherapeutic effect.And promote inflammation suppression factor of IFN-gammasecretion and expression, inhibiting proinflammatory factor beta IL-1, secretion andexpression of TNF alpha, is probably clear lung detoxification mechanism of action ofinfluenza virus FM1.