| Objective:Preparation of MCI model in rats by ligation of right commo n carotid artery method with the corresponding drug, after5we eks of treatment, observed the rat model of behavior, and deter mination of NO content and TNOS activity in the cortex of the r ats. By contrast with other groups, impact assessment of modifi ed Ditan Decoction on learning and memory abilities of MCI mode1rats.Methods:1.The preparation group and model in ratsTwo months SPF60SD rats, male and female half, using the stochastic method is divided into5groups, namely blank group, model group, control group, the flavored polyester phlegm deco ction group, nim ground group, each group of12. Except the blan k group, the rest of the group with the right common carotid ar tery ligation method preparation of MCI model, the control grou p only to find the right but not common carotid arteries ligati on. Flavored polyester phlegm decoction group, nim horizon in5weeks began to fill the stomach, the remaining three groups of saline lavage. Lavage5weeks after the start line detection rel ated indicators. Flavored polyester phlegm decoction (tiannanxin g10g. faxia10g. zhishi lOg. fuling15g. juhong10g. shichangpu6g. renshen6g.zhuru lOg. gancao6g.shengjiang5. danshen lOg.chuanxi ong lOg)2. Each group rats behavior detectionThe Morris water maze. Results:the Morris water maze in Ⅰ, Ⅱ, Ⅲ, Ⅳ four quadrants, the rats for the wall from the first quadrant cast into the water, record the time limit (2minutes) to find the platform within the time (latency). Lasted5days, daily2times (including4days ago to training period, fifth days for the detection period).Experimental research of Morris water maze spatial:location and navigation after test, the Morris water maze in platform out, still with the same navigation training into the water will be cast into the water rat, recording the time limit (2minutes) marched trajectory, rats were observed across the placing platform position, near the platform area number of time. Lasted4days, daily2times (including3days ago to training period, fourth days for the detection period).Object recognition experiment:test during the training per iod into A set of objects in the oven, put in the rats of dista nee between two objects, quickly close test box, recording time rats exploring on each object (rats with nose contact objects, or rat nasal tip1cm distance object range, if the rats on ob jects or only travel outside of the object1cm is not include d).Period of the test, the test in one of the objects, the orig inal location in the B set, an object of the rats in the same w ay, record rats to search for fresh object and be familiar with the time. Record fourth big rat total search time, fifth day fo r detection period, record the rats to find fresh object takes time (N) and to seek for familiar objects takes time (F), and o n the basis of the formula to calculate the discrimination inde x (discrimination index, DI).3. The NO content in the rat cortex and TNOS vitality detectionBehavior after the test, beheaded kill each group rats, pla ce the mouse head Yu Bingpan, step by step from the hippocampus and cortex, hippocampus and cortex respectively take out again, inserted in different markers of EP, quickly into the liquid n itrogen staging in the bottle, then transferred to-80℃refr igerator kept in quarantine.Detection, apply adequate amount to the cortex, with nine times the amount of normal saline grindi ng, and then put the grinding fluid in desktop centrifuge at3000RPM speed centrifugal10minutes, drain on clear liquid, in4℃refrigerator keep them waiting for inspection. Then built s trictly according to the nanjing institute of biological engine ering of nitric oxide and nitric oxide synthase kit detection m ethod to detect the contents of NO in cortex and TNOS vitality.Results:1. The test of Morris water maze, the rats compared to the average escape latency, removed the platform across the platform location number. The model group compared with the control group, the rats in the model group compared with blank group, the average escape latency time prolong, across the platform position number less than the blank group, there was a statistically significant difference (P<0.05). The model group compared with sham operation group, while the sham operation group rats escape latency time shortened than the model group, the number of crossing platform position compared with the model group, but the two difference was not statistically significant (P>0.05). Modified Ditan Decoction group compared with the model group, while the modified Ditan soup group rat escape latent period of time compared to the model group reduced, across the platform position number increased in comparison with the model group, but the two difference was not statistically significant (P>0.05). Modified Ditan Decoction group and nimodipine group, al though modified Ditan Decoction group were the average escape latency, removed the platform across the platform position number have the tendency to increase, but the difference was not significant, there was no statistical significance (P>0.05).2. In object recognition and detection, the model group compared with the control group, the old and the new object discrimination index of rats in the model group decreased than control group, the total search time, there was a statistically significant difference (P<0.05). The model group compared with sham operation group, although the index of discrimination of old and new objects in the sham operation group rats were significantly increased, the total search time prolonged, but the two difference was not statistically significant (P>0.05). Modified Ditan Decoction group compared with the model group, modified Ditan Decoction group rat total search time compared with the model group, the old and the new extension of object discrimination index rose, the two have statistically significant difference (P<0.05). Modified Ditan Decoction group and nimodipine group, although the index of discrimination of the old and the new object of modified Ditan Decoction group rats increased, total seek time prolonged, but the difference was not significant, there was no statistical significance (P>0.05).3. Display the contents of NO, TNOS activity in the cortex of rats in each group:the test results of the model group compared with the control group, NO content and TNOS activity in the cortex of rats in the model group than in blank group increased, there was a statistically significant difference (P<0.05). The model group compared with sham operation group, sham operation group rats cortex NO content and TNOS activity decreased compared with control group, there was a statistically significant difference (P<0.05). Modified Ditan Decoction group compared with the model group, modified Ditan Decoction group rat cortical NO content and TNOS activity decreased compared with the blank group, the two have statistically significant difference (P<0.05). Modified Ditan Decoction group and nimodipine group, there was no significant difference in NO content and TNOS activity in the cortex of rats in the two groups, which was not statistically significant (P> Conclusion:In this experiment, the rat model of MCI was prepared by ligating the right carotid artery method, intragastric administration related indicators5weeks. Research by comparison of NO content and TNOS activity of modified Ditan Decoction on the behavior of MCI model rats and the effect of cortex, found that modified Ditan Decoction can reduce MCI average escape latency model rats, increase the rat across the platform position, reduce the number of rat cortex NO content and TNOS activity, up to effectively protect the nerve cells in the brain, so as to improve the spatial memory ability of MCI rats, play to block or delay the decline in learning and memory process effect. The learning and memory function of modified Ditan Decoction on rat model of NCI effect. |
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