Effect Of Dihydroartemisinin Combined With Cisplatin On Immune Function Of Tumor Bearing Rats

ObjectiveAt the base of establishing an animal model of Fischer344rat poorly differentiatedepithelial ovarian cancer, the purpose is to study the impact of different doses ofDihydroartemisinin carboplatin on immunosuppression of tumor-bearing rats.So asto explore whether there is a theoretical basis to prove Dihydroartemisinin has helpedto the cure of Cisplatin-resistant ovarian cancer.Methods1. Poorly differentiated epithelial ovarian cancer cell lines NUTU-19in vitro,then cellsuspension is injected in the Fischer344subcutaneously in rats to establish an animalmodel of human ovarian cancer xenografts.2. The40Fischer344rats of successly Modeling were randomly divided into fivegroups which were the carboplatin group,the control group,the carboplatin+artemisinin high dose group,the carboplatin dose artemisinin group,the carboplatin+Green artemisinin low-dose group and so on.The rats were all administered by theway of intraperitoneal injection. After10days of administration, they weresacrificed.Then removed the tumor, observed tumor morphology. After this,weighthe weight of tumor tissue,and calculated inhibition rate,given a histopathologicalexamination under the microscope.Measured ymphocytes conversion feature,thendetected the changes of the serum IL-2and IFN-γby ELISA.Result1.Effects of Carboplatin+Dihydroartemisinin on rat tumor growth:the inhibition tumor rates of Carboplatin+Dihydroartemisin in high dose group,middle dose groupand low dose group were compared to30.26%,47.32%and62.13%.The inhibitiontumor rate of carboplatin group is28.14%.Compared with the carboplatin group andthe carboplatin+artemisinin group,the inhibition tumor rate of the carboplatin+artemisinin group increased significantly. The differences had statisticallysignificant (P<0.001).2. Monitor of transplanted tumor histopathology: By observing the carboplatin groupand the carboplatin+artemisinin group under the microscope,I had found the largervolume of tumor cells,cytoplasm loose, nuclei hyperchromatism, different levels ofnuclei condensation, nuclei fragmentation and nuclei fusion. Focal necrosis is visiablein cells.3.Compared with Carboplatin+Dihydroartemisin in middle dose group, low dosegroup and Carboplatin,the differences between rat T/B lymphocyte transformationrate and serum IL-2and IFN-γ leves have no statistically significant(P>0.05). Ratlymphocyte transformation rate and serum IL-2and IFN-γ leves of Carboplatin+Dihydroartemisin in high dose group are obviously higher than that of Carboplatingroup(P<0.001).Conclusion1Dihydroartemisinin conmbined with Carboplatin has a inhibition function on thegrowth of rat ovarian cancer xenograft.Its combined effects is stronger thanCarboplatin alone,which enhance the sensitivity of Carboplatin.2Dihydroartemisinin conmbined with Carboplatin has a intensive function on ratimmune function. By improving the transformation function of rat spleen lymphocytes and the levels of rat serum IL-2and IFN-γ, it may reduce the riskof recurrent ovarian cancer and lymph node metastasis.