Inhibitory Effect And Mechanism Of Dihydroartemisinin Combination With Carboplatin On Ovarian Cancer Cell Line SKOV₃

Objective:To investigate the inhibitory effect and the mechanism of dihydroartemisininwhen used alone or in combination with carboplatin on ovarian cancer cell lineSKOV3.Method:SKOV3cells were divided into four groups:the negative control group、dihydroartemisinin group、carboplatin group and combination group.The cells weretreated for24h,48h and72h and MTT assay was used to test the inhibition of cellgrowth. The formation rate of SKOV3cells colones was evaluated by colony assayafter48h treatment. Drug effects on SKOV3cells apoptosis was detected by flowcytometry after48h treatment. The expression of VEGF mRNA and protein wasassayed by RT-PCR and Western blot respectively after48h treatment.Result:MTT assay results showed that the drug groups all inhibited the growth ofSKOV3cells in a dose-dependent manner, and significant difference existed betweenconbination group and negative control group (p＜0.05). Colony assay revealed thatthe drug groups all inhibited the colony formation of SKOV3cells,and the inhibitioneffect on SKOV3cells of dihydroartemisinin combined with carboplatin was strongerthan that of the single-drug groups(p＜0.01). The result of flow cytometry showedthat the drug groups all trigger the apoptosis of SKOV3cells and the apoptosis ratewas higher in combination group than in other groups(p＜0.05). Both the result ofRT-PCR and Western blot showed that the mRNA and protein expression level ofVEGF in SKOV3cells was decreased and the significant decreased in combinationgroup(p＜0.05).Conclusions:Dihydroartemisinin enhanced the inhibitory effect of carboplatin on SKOV3cells and the mechanism of that may be through down-regulating the proteinexpression of SKOV3cells.