The Influence Of Drugs And Alcohol On The Mesenteric Microcirculation Of Experimental Diabetic Rats

Objective: to study the influence of norepinephrine (NE), sodium nitroprusside (SNP)and alcohol on the experimental diabetic rats mesenteric microcirculation，reactivity.Methods78male SD rats and78experimental diabetic rats, weighting220~250g.Imaged with the BI-2000medical image analysis system, the mesentericmicrovascular diameter of anesthesia rats was recorded in different localconcentration of NE (10-12~10-4mol/L), SNP (10-10~10-4mol/L) and Alcohol(0.025%~3.2%) and different intragastric of Alcohol(0.375%～48%),and to observethe influence of NE between the control group and STZ group when the mesentericmicrocirculation hatched by phentolamine and the influence of SNP between thecontrol group and STZ group when the mesenteric microcirculation hatched byL-NAME.Part1. The influence of different local concentration of NE (10-12~10-4mol/L) addto the Normal and experimental diabetic rats mesenteric microcirculation, to observethe reactivity of NE on rat’s mesenteric microcirculation. The influence of NEbetween the control group and STZ group when the mesenteric microcirculationhatched by phentolamine.Part2. The influence of different local concentration of SNP (10-10~10-4mol/L) addto the Normal and experimental diabetic rats mesenteric microcirculation, to observethe reactivity of SNP on rat’s mesenteric microcirculation. The influence of SNPbetween the control group and STZ group when the mesenteric microcirculationhatched by L-NAME.Part3. The influence of different local concentration of Alcohol (0.025%~3.2%) anddifferent intragastric of alcohol(0.375%～48%) to the Normal and experimentaldiabetic rats mesenteric microcirculation, to observe the reactivity of Alcohol on rat’smesenteric microcirculation.ResultsPart1. The mesenteric microcirculation of rats was contracted by NE concentrationdependently.The EC50of NE on the mesenteric arteriole/venule was(4.14±0.35)×10-9mol/L、(2.95±0.48)×10-8mol/L. The microvascular response to NE decreased significantly in experimental diabetic rats compared with the normalgroup’s (P<0.05). The EC50of NE on the mesenteric arteriole/venule was(5.39±0.29)×10-8mol/L、(4.78±0.39)×10-8mol/L. The influence of NE on thecontrol group was stronger than STZ group when the mesenteric microcirculationhatched by phentolaminePart2. The mesenteric microcirculation of rats was relaxed by SNP concentrationdependently. The EC50of SNP on the mesenteric arteriole/venule was （2.44±0.32）×10-8mol/L、(5.85±0.40)×10-8mol/L. The microvascular response to SNP decreasedsignificantly in experimental diabetic rats compared with the normal group’s (P<0.05).The EC50of SNP on the mesenteric arteriole/venule was (6.66±0.43)×10-8mol/L、(8.78±0.59)×10-8mol/L. The influence of SNP on the control group was stronger thanSTZ group when the mesenteric microcirculation hatched by L-NAME.Part3. The mesenteric microcirculation of rats was relaxed by Alcoholconcentration dependently. The EC50of alcohol on the mesenteric arteriole/venule was（0.13±0.02）%、（0.17±0.02）%,The EC50of intragastric alcohol on the mesentericarteriole/venule was （2.81±0.22）%、（2.85±0.21）%. The microvascular response toalcohol decreased significantly in experimental diabetic rats compared with thenormal group’s (P<0.05). The EC50of alcohol on the mesenteric arteriole/venule was(0.25±0.03)%、（0.32±0.03）%, The EC50of intragastric alcohol on the mesentericarteriole/venule was (2.79±0.23)%、（3.25±0.23）%.Conclusion1.The microvascular response to NE decreased significantly inexperimental diabetic rats.2. The microvascular response to SNP decreasedsignificantly in experimental diabetic rats.3. The microvascular response to alcoholdecreased significantly in experimental diabetic rats.