Sufentanil Effect The Endothelium Of The Thoracic Aortic On Type2Diabetic Rats

Objective:the different concentration of Sufentanil on type2diabetic rats in vitroeffect of thoracic aortic endothelial.Method:16healthy male Wistar rats were randomly grouped,Normal rats group(N,n=8), with common feed;Diabetic rats group (D,n=8), induced diabetes withintraperitoneal injection of urea. After building a successful animal model,eachthoracic aorta of rats rapidly is separated and taked4arterial ring,carefully removedthe perivascular connective tissue,every2-4mm long, and immediately put into thecontaining k-h precooling of nutrient solution in order to maintain the activity ofvascular ring in the blood vessels, the nutrient solution ensures sufficient oxygensupply for continuous ventilation with95%O2+5%CO2gas mixture,4period ofvascular ring were given saline groupC、Sufentanil7×10-11mol L-1groupS1、2×10-10mol·L-1group S2、1×10-9mol·L-1group S3.Vascular ring tensionmeasuring device are connected at one end, the other end using vitro vascular ringhatch of the perfusion method in the tube. Contraction vascular effects of KCl,first ismeasured loop balance tension G1;then NE is strong vasoconstrictor drugs,the ring iswashed off and gived accumulation of different concentrations of NE (endothelialdependent shu vascular material) to measure vascular tension G2,calculation ofG2/G1X100%to reflect endothelial function;Then by ELISA, enzyme-linkedimmunoassay, groups of vascular rings organization for grinding take on centrifugalclear liquid，antibody-antigen-enzyme mark antibody complex is producted byREDOX reaction，a standard curve calculates NO NOS expression in rat vascularring under450nm wavelength absorbance value (OD). it observes the quantitative NO NOS expression of32vascular ring after the different concentrations ofsufentanil in normal group, the diabetic rats group in a similar way,the high lever ofsufentanil in normal and diabetes groups is respectively analyzed by vascular ringfor NO NOS expression, to reflect the effect of vascular endothelialResult：1. Compared with the NC， NS2、 NS3vascular ring contraction amplitudereduction,Tissue homogenate NO、NOS expression increased (P<0.05).2. Compared with the DC，DS1、DS2vascular ring contraction in DS3group werelower, tissue homogenate NO、NOS expression were higher (P<0.05);Comparedwith the DS1，DS2、DS3group of vascular ring contraction amplitude reduction,higher organization NO、NOS expression；The DS3group had a significantlylower contraction of vascular ring than the DS2，organization NO、NOSexpression significantly increased (P<0.01).3. Compared with the DS3, NS3group and NO、NOS expression with vascular ringtension difference (P<0.05).Conclusion：Different concentrations of sufentanil on diabetic rats in vitrothoracic aorta vascular has inhibitory effect of contraction， High levels wassignificant，The mechanism is associated with high NO expression of endothelialfactor， The NOS synthase is the pivotal issue enzyme of the biologicaltransformation.