Maternal In Utero Exposure To Di-(2-ethylhexyl) Phthalate Affects The Heart Of Adult Female Offspring

ObjectiveDi (2-ethylhexyl) phthalate(DEHP)is generic industrial plasticizers, which is alsoone of causes of the importance of chemical substances that may lead to metabolicdisorders and circulatory disorders in children.In this paper, Sprague-Dawley (SD)pregnant rats were exposed to DEHP in utero.And then measure cardiac function andevaluate myocardial pathology of the postnatal offspring of female rats in order toinvestigate effects of cardiac injury in SD female offspring rats.MethodsWe established SD pregnant rat model of intrauterine exposure to DEHP.Since thefirst day of pregnancy until delivery, SD pregnant rats were fed corn oil,low dose (10mg.kg-1.d-1) DEHP or high-dose (100mg.kg-1.d-1) DEHP at14:00. We Observed thegeneral condition (body weight),cardiac hemodynamics and myocardial histopathologyof the offspring of female rats in each group.Results1. The weight of female offspring rats on the born (d0) and21days after born (d21):Compared with the control group, the weight of DEHP exposure group wassignificantly increased, with statistical significance (P <0.05); the weight of femaleoffspring rats at70days after birth (d70): compared with the control group, the weight of DEHP high-dose group was significantly gained,with statistically significant (P<0.05),while no significant difference was in the low-dose group (P>0.05).2.Fot high-dose group,Heart rate(HR) and left ventricular end-diastolic pressure(LVEDP) was significantly higher than the control group and low dose group.And leftventricular systolic pressure (LVSP),left ventricular maximum rate of rise (+dp/dtmax)and left ventricular maximum rate of decrease (-dp/dtmax) was significantly lower thanthe control group and low dose group, the difference was statisticallysignificant (P <0.05).3.Light microscope of high dose group in female rats offspring demonstratedcardiomyocytes hypertrophy,myocardial fiber disarray, a large number of myocardialcells karyopycnosis and stained; electron microscopy of that showed a lot of myocardialcells karyopycnosis and stained, myocardial fiber disorder and fracture arrangement,most of mitochondrial cristae broken and blurred. However, no obvious change wasfound in the control group and low dose DEHP group.Conclusion1. Maternal in utero exposure to DEHP can cause weight gain in the female offspringrats, hemodynamic abnormalities (diastolic function and systolic function was subjectedto suppress the influence) and myocardial structure disorder and destruction.2. Maternal in utero exposure to high doses (100mg/kg) DEHP can cause ventricularhemodynamic abnormalities,leading to systolic and diastolic dysfunction.3. Light and electron microscopy showed that female offspring rats of DEHPexposure group had various damages in cardiac microstructure,with particularlysignificant in high-dose group. Myocardial cell structures damaged,cardiac functionmight decline.