Study Of Relationship Between SP-A1Polymorphism And Neonatal Respiratory Distress Syndrome In Preterm Infant

Objective: To investigate surfactant protein A1(SP-A1) genepolymorphism and its relationship with respiratory distresssyndrome(RDS) in preterm infant.Methods: Using a case-control cohort design, we recruit34preterminfants with respiratory distress syndrome(RDS) as study cohort and44preterm infants without RDS as control. ALL infants are Han ethnic andfrom Guangxi region. Both RDS and non-RDS groups were matched withgestational age and gender. We used TaqMan real-time polymerasechain reaction technology to detect five single nucleotidepolymorphisms(SNP) of SP-A1(rs1059047、rs1136450、rs1136451、rs1059057、rs4253527) and used HESIS software to analysis allelefrequency and haplotypes frequency and compare them between RDS andnon-RDS groups.Results:1. We totally identified14haplotypes in general group,including case and control. Of which,4were unassigned and3werenovel.6A2and one of unassigned（CGAAC）had the highest frequency. Compared to control.6A2frequency in case group was significant higherthan that in control group, while6A18frequency in control group wassignificant higher than that in case group.2. In subgroup of gestation ageof smaller than32weeks, we identified6haplptypes and two of whichwere unassigned which also seen in general group. Case group6A3and6A11to the highest frequency, but no significant different of eachhaplotype frequency be found between case group and control group.3.In subgroup of gestation age of larger and equal to32weeks, weidentified totally16haplotypes and6were unsigned. Among these, allunassigned haplotypes had a frequency lower than0.15, except(CGAAC). Similar to the subgroup of gestation age of smaller than32weeks, no significant difference of each haplotye frequency be foundbetween case group and control group.Conclusions:1. Contribution of variant in SP-A1to high risk ofRDS is still unknown.2. SP-A1haplotype presents differentcharacteristic in different ethnic and race population.