The Clinical Study Of Serum Cytokines In Patients With Secondary Tuberculosis And Nontuberculous Mycobacteria Disease

【Objective】Tuberculose is a chronic infectious disease induced by mycobacterium tuberculosisinfection, which is mainly occurred in the lung tissue, and is considered to be a most ancientand most distributed disease in the world. Although secondary tuberculosis accounts for amajor rate of patients with tuberculosis, the serum inflammatory cytokines in patients withsecondary tuberculosis and nontuberculous mycobacteria disease are largely unknown.Therefore, our study is aimed to investigate the serum IL-1, IL-2R, IL-6, IL-8, IL-10, TNF-αand PCT levels in patients with secondary tuberculosis and nontuberculous mycobacteriadisease and their clinical significance.【Methods】The serum contents of IL-1, sIL-2R, IL-6, IL-8, TNF-α and IL-10were examined in71patients with secondary tuberculosis and21controls using ELISAassay. The serum contentsof IL-1, IL-2R, IL-6, IL-8, IL-10, TNF-α and PCT were examined in11patients withnontuberculous mycobacteria disease and14controls.【Results】1. The serum sIL-2R and TNF-α levels in patients with secondary tuberculosissignificantly increased compared with the controls (P<0.01), however, the serum IL-1andIL-10contents were not greatly affected (P>0.05). Further analysis showed that changes ofserum sIL-2R in patients with secondary tuberculosis correlated with the number of acid fastbacilli (P<0.05), and the IL-10contents in patients of secondary tuberculosis with cavitygreatly increased compared with those without cavity (P<0.05). 2. The serum TNF-α levels in patients with nontuberculous mycobacteria diseasesignificantly increased compared with the controls (P<0.05), however, the serum IL-1,IL-2R, IL-6, IL-8, IL-10, and PCT were not greatly affected (P>0.05). Further analysisshowed that changes of serum IL-1, IL-2R, IL-6, IL-8, IL-10, TNF-α and PCT in patientswith nontuberculous mycobacteria disease did not correlated with cavity (P>0.05).【Conclusion】1. The serum TNF-α, sIL-2R and IL-10levels may be a set of valuable marks for thediagnosis and progression of secondary tuberculosis infection.2. The serum TNF-α level is significantly increased in patients with nontuberculousmycobacteria disease, which may contribute to the diagnosis of nontuberculousmycobacteria disease infection.