Expression And Clinical Significance Of MCM5and P16^INK4A In Cervical Squamous Cell Carcinomas And Precancerous Disease

Object:To investigate the expression of MCM5and P16INK4A in cervical tissues of differential pathological types, especially in cervical intraepithelial neoplasia(CIN) and cervical carcinomas, in order to analyse the relationship of MCM5and P16INK4A with clinical and pathological features of cervical carcinoma and the relevance of each other. To explore the role of MCM5and P16INK4A in the progression from CIN to cervical tumour. Furthermore to provide effective markers in screening, prognosis of cervical cancer.Methods:Real-time fluorescence quantitative PCR and immunohistochemical staining SABC method were used respectively to examine the expression of mRNA and protein of MCM5and P16INK4A in40cases of cervical squamous cell carcinoma,11cases of low-grade lesions (CIN I),15cases of height-grade lesions(CIN Ⅱ-Ⅲ) and15cases of normal cervical tissues. The expression of of mRNA and protein of MCM5and P16P16INK4A was compared in different cervical tissues. To analyze the relationship of MCM5and P16INK4A with age, clinical stage and the differentiation of cervical cancer, the relevance of each other.Results:The expression of mRNA and protein of MCM5and P16INK4A was significantly up-regulated in carcinomas than in CIN Ⅰ, CIN Ⅱ-Ⅲ and normal cervical tissues. The overall difference in the expression of mRNA and protein MCM5and P16INK4A in the normal cervix, CIN Ⅰ, CIN Ⅱ-Ⅲand cervical cancer groups is highly statistically significant (P<0.01).1. The expression of MCM5and P16INK4A mRNA and protein in different cervical tissue1.1The expression of MCM5mRNA in different cervical tissue:The expression of MCM5mRNA added with the progress of cervical lesion. The expression of mRNA of MCM5was significantly up-regulated in carcinomas, CIN Ⅰ and CIN Ⅱ-Ⅲ than normal cervical tissues, there were significant difference with each other (P<0.05). however, there was no significant difference between CIN Ⅰ and CIN Ⅱ-Ⅲ(P>0.05)。The expression of MCM5protein in different cervical tissue:The expression of MCM5protein gradually increased from normal cervical tissue to cervical cancer. MCM5positive expression rates in15normal cervical tissue,11CIN Ⅰ,15CINⅡ-Ⅲ and40cervical squamous cell carcinoma samples were20%,63.6%,73.3%,97.5% respectively. The expression of protein of MCM5was significantly up-regulated in carcinomas than CIN Ⅰ,CIN Ⅱ-Ⅲ and normal cervical tissues, the expression of MCM5showed significant difference between cervical cancer and CIN Ⅰ,CIN Ⅱ-Ⅲ and normal cervical tissues(P<0.05).1.2The expression of P16INK4A mRNA in different cervical tissue:The expression of P16INK4A mRNA gradually augmented with the headway of the cervical lesion. There were significant difference when the group of cervical cancer compared with the group of CIN I and normal cervical tissues(P<0.05),but there was no importance between the group of cervical cancer and CIN Ⅱ-Ⅲ (P>0.05); Compared the group of CIN I with the group of normal cervical tissues, there was also no difference (P>0.05), while there was also importance between the group of CIN Ⅰ and CIN Ⅱ-Ⅲ (P<0.05). The expression of P16mK4A protein in different cervical tissue: MCM5positive expression rates in15normal cervical tissue,11CIN Ⅰ,15CIN Ⅱ-Ⅲ and40cervical squamous cell carcinoma samples were respectively0%%27.3%、86.7%、95.0%. The expression of P16INK4A protein showed significant difference in CIN I and normal cervical tissue, CIN Ⅱ-Ⅲ and cervical cancer(P<0.05), but there was significant difference in cervical cancer and normal tissue, cervical cancer and CIN I. There was also importance between CIN I and CINⅡ-Ⅲ (P<0.05)2. The relation of mRNA and protein of MCM5and P16INK4A in invasive carcinoma of cervix to the factors related to clinical pathologythere was association between MCM5expression and clinical stage, the differentiation (P<0.05). however, there was no association between MCM5expression and age(P>0.05). there was association between P16INK4A expression and the differentiation of cervical cancer (P<0.05). but the expression of P16INK4A showed no significant relation in different age and clinical stage.(P>0.05)The correlation analysis of the expression of MCM5and P16INK4A in cervical cancer tissue:In invasive cervical cancer, the expression of MCM5and P16INK4A showed positive correlation.(r=0.538, P<0.01)Conclusion:The expression of MCM5and P16INK4A increased with the progress of cervical lesion. There was a significant difference in different cervical lesion; The expression of MCM5and P16INK4A had correlation with the grades of histological in cervical squamous carcinoma, and there was a positive correlation between MCM5and P16INK4A in tissue of cervical cancer. There was an important meaning of predicting outcome of cervical intraepithelial neoplasia and the progression and prognosis of cervical cancer by union examining MCM5and P16INK4A.