The Rapid Trace Detection Of Porphyromonas Gingivali In Saliva

Periodontosis is one of the most common infectious diseases in oral medicine caused by anaerobic bacteria, including Porphyromonas gingivalis, Actinobacillus actinomycetemcomomitans, Tannerella forsythia. The bacteria whatever in phylotypes or number that can be detected by traditional cultivation method in fact is much lower than the actual existence. However, the most of the molecular biology techniques can only be used for oral pathogenic bacteria qualitative research. Quantitative real-time polymerase chain reaction(RT-qPCR) has achieved the quantitative study of DNA template with high sensitivity, specificity and reliability. Saliva is important body fluids which has complex components, moreover it is convenient when we collect samples from patients. Therefore, saliva diagnosis will have a great application promise in the early detection of periodontal and caries disease, efficacy judgement and prognostic evaluation.Object:In this study, we detected the amount of Pg in saliva of periodontitis patients and periodontal healthy subjects by Pg specific genes Arg-gingipain adopting SYBRgreen real-time fluorescent quantitative PCR technology. We have assessed differences in the Pg number between all groups. The purpose is to investigate the distribution and pathogenic mechanism of Pg in periodontitis to provide the basis for diagnosis, treatment and curative effect evaluation of the disease.Methods:We have examined quantity of Pg in saliva of20chronic periodontitis and20periodontal health using SYBRgreen real-time quantitative PCR. T test has been brought to analyze the differences of Pg engraftment among all groups.Results:In samples of chronic periodontitis, the average Log number of Pg was1.01■0.77and positive rates was85%, that in male and female were1.00±0.76、1.02±0.83, respectively. While in samplels with periodontal healthy subjects, the average Log number of Pg was0.35±0.11, and positive rates was10%, that in male and female were0.36+0.12、0.34±0.10, respectively. The difference of saliva Pg number between chronic periodontitis and periodontal healthy subjects was significant in statisties (P<0.05). However, there was no significant difference between male and female of each group in Pg amounts (P>0.05).Conclusion:Saliva diagnosis is a reliable way of microbial detection, so that may be helpful to clinical disease auxiliary diagnosis. The reseach proved that Pg is closely related to the occurrence of periodontal disease, and we found that the detection of Pg has no obvious correlation to gender.