An Early Stage Observation Of Tissue-engineered Periosteum In Repairing Rabbit Radial Large Segmental Defect, And The Effects Of BMP-2on Tissue-engineered Periosteum Osteogentic Capacity In Vitro

Objective:To compare the early effect of tissue-engineered periosteum(TEP) and deproteinised bone in repairing rabbit large segmental bone defects.Method:The bone marrow mesenchymal stem cells(BMSCs) obtain from New Zealand rabbits and cultured in vitro were seeded on porcine small intestinal submucosa(SIS) to fabricate TEP. Totally24New Zealand rabbits of12weeks old were accepted osteotomy surgery to careate large segmental bone defect(30mm) model in unilateral radius. Those rabbits were randomly divided into three groups, there were each8rabbits in group A,B and C. The rabbits of group A were given a TEP transplant in the bone defect area,while group B were given a SIS transplant.and the group C received a deproteinised bone grafts in defect area. At4weeks after operation, x-ray examination were taken to analyze bone defect reconstruction, then the animals were sacrificed for histological examination.Result:In group A, bone defects were radiographically and histologically cured with the new bone tissue formation, while none of the defects healed in group B and C.Conclusion:Tissue-engineered periosteum can be used to heal the large segmental bone defect of radius in allogenic rabbits,and its performance is much better than deproteinised bone. Objective:To inspect the effects of recombinant human bone morphogenetic protein-2on osteogentic capacity in vitro of tissue-engineered periosteum which were constructed by porcine small intestinal submucosa(SIS) and the bone marrow mesenchymal stem cells(BMSCs).Method:150ul1x105cell/ml New Zealand rabbits BMSCs cultured and osteogenic induced in vitro were seeded on0.8cm2round SIS to manufacture the tissue-engineered periosteum(TEP).The TEPs were divided into four groups and cultured with rhBMP-2of different concentrations (100ng/ml、50ng/ml、10ng/ml and Ong/ml).Then growth curves of BMSCs on TEP of each group were detected by MTT, and the micro-structure of TEP were scaned by electronic microscopy at7d. Moreover,the the ALP activity and osteocalcin content of each group TEP were determined by ELISA kit at several different time point.Result:Growth curves, ALP activity and osteocalcin content show that the TEP with rhBMP-2get significantly better performance than none, and there are a positive correlation between rhBMP-2doses and the TEP’s osteogentic capacity.Conclusion:rhBMP-2can enhance the TEP’s osteogentic capacity in vitro,so it can be used to improve the TEP.