The Experimental Study Of Tissue-engineered Artifical Periosteum To Repair Rabbit’s Scapula Defect

Objective:The aim of this study was to discuss the feasibility and effectiveness of Tissue-engineered periosteum(TEP),which constructed with bone marrow mesenchymal stem cells (BMSCs) and porcine small intestinal submucosa (SIS), for the reconstruction of large bone defects in allogenic rabbit body through intramembranous ossification. TEP was made up SIS and osteoblasts which were induced from BMSCs. then preparation of deproteinized bone.Methods:The scapula was cut off except the glenoid cavity and part of the shoulder neck. The bone defect were created with TEP (group1), DPB (group1)and compound which was made up TEP and DPB (group3). X-ray radiography and Histological examination were performed at4,8and12weeks.ResuIts:The outcomes show newly formed bone were observed at each time in both Group1and Group3. The massive and irregular scapula defect was Repaired at12weeks in both groupsas well as a few calluses were observed in group3. furthermore, blood vessels were observed by Histological examination in group1and Group3.Conclusion:The study shows that Tissue engineering periosteum can repair the rabbit scapula massive and irregular defect.