| Objective: To study the effect of artificial synthetic E-selectin on BBB permeabilityand infarction and edema volume after cerebral ischemia reperfusion injury in rats. Theimprovement of Zea Longa was used to establishe focal cerebral ischemia reperfusioninjury model.Methods:90male SD rats were randomly divided into sham-operation group (groupA), ischemia-reperfusion group (group B) and artificial synthesized E-selectin treatedgroup (group C), with30rats in each group. The middle cerebral artery occlusion (MCAO)referring Longa’s method and added some refinements was used to establish the focalcerebral ischemia model.10minites before building the models, each rat of group C wereinjected into femoral vein with artificial synthetic E-selectin (10mg/kg). Each rat of theexperiment were injected into femoral vein with2.5%evans blue (0.2mg/kg) before killed.To evaluation the effect of artificial synthetic E-selectin on BBB permeability all rats weretested by Longa neurological behavior, measured infarction and edema volume by MRI,determination Content of EB by enzyme-labeled instrument at different times after reperfusion(3h,6h,24h,48h,72h).Results:（1）Neurological deficit was not found in group A, while found in group Band C. Some times after injury the neurologic impairment score of group C was lower thangroup B;（2）Not any infarction and edema was found in group A. The infarction andedema volume of group C was significantly lower than group B (P<0.05).(3) No obviouschanges of the EB contents of group A could be found at different times of reperfusion. The group B and C had a similar variation tendency: at3h of reperfusion the EB contentsbegan to increase,6h continued to rise, reached the peak at24h and began to decrease at48h, still elevated at72h. The EB contents of group C was significantly lower than group Bat different times of reperfusion (P<0.05).Conclusions:1. The cerebral ischemia reperfusion injury in rats can lead to increasedpermeability of the blood-brain barrier;2. The artificial synthetic E-selectin can reduce thecerebral infarction and edema volume caused by cerebral ischemia reperfusion injury, canmitigate the damage leads to increased permeability of the blood-brain barrier, has someprotective effects of blood-brain barrier. Objective: To study the effect of the artificial synthetic E-selectin on expression andlocation configuration of tight junction proteins (occludin, ZO-1) after cerebral ischemiareperfusion injury in rats, and investigate the possible mechanism of protective effects ofblood-brain barrier.Methods: The middle cerebral artery occlusion (MCAO) referring Longa’s methodand added some refinements was used to establish the focal cerebral ischemia model.90male SD rats were randomly divided into sham-operation group (group A),ischemia-reperfusion group (group B) and artificial synthesized E-selectin treated group(group C), with30rats in each group. Brain tissue specimensat were extracted at differenttimes after reperfusion (3h,6h,24h,48h,72h). The expression and location configurationof occludin and ZO-1was assayed by using immunohistochemical staining, and theexpression of occludin and ZO-1was measured by Western blot.Results: Immunohistochemical staining:（1）A large numbers of positive cells ofoccludin and ZO-1were observed along the capillaries in gourp A;（2）The amount ofexpreesion of occludin and ZO-1of group B was began to decreas at3h of reperfusion,reached the lowest at24h, and increased at72h, but still lower than those of group A. Theexpression shape of occludin become fracture at24h of injunry;（3）The amount ofexpreesion of occludin and ZO-1of group C was richer than group B but lower than group A, and the continuity of expression was better than group B but worse than group A at24hof reperfusion.Western blot analysis:（1）The expreesion of occludin and ZO-1of group A did notsignificantly variation（;2）The expreesion of occludin and ZO-1of group B had the similarvariation tendency: began to decrease at3h of reperfusion, continued to reduce at6h,reached the lowest at24h and began to increase at48h, still lower than gourp A at72h;（3）The expreesion of occludin and ZO-1of group C had the similar variation tendency ofgroup B, but the expreesion levels of occludin and ZO-1higher than group B (P<0.05).Conclusions:1. The cerebral ischemia reperfusion injury in rats can reduce theexpreesion of occludin and ZO-1proteins;2. The artificial synthetic E-selectin canalleviate the reduce of occludin and ZO-1proteins and maintain the structure of occludin,this may be one of the BBB protective mechanisms. |
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