ODN MTO1Mediate The MRNA Expression Of Inlfammations After Murine Macrophage Infection With Porphyromonas Gingivalis

Periodontitics is a chronic disease characterized by destruction of the supportingtissues. Periodontitis is the main reason for the loss of our adult teeth. The harm notonly affects chewing, the basic function such as pronunciation, but also severelyaffected with the quality of life of patients. How to improve the level of diagnosisand treatment of periodontal disease prevention are the enormity of the task andhistorical mission to the dental professionals in current. In recent years, the studyfound that Periodontitics is a chronic disease which is caused by plaquemicroorganisms.The periodontal pathogens can excitate the periodontal innateimmune response which cause periodontal tissue damage and bone resorption.Studieshave confirmed NLRP3as an important component of the innate immune is closelyrelated to the occurrence of periodontal disease development.So the mechanism ofbone resorption,in view of its pathogenesis, we will find a way to inhibit alveolarbone absorption and to promote alveolar bone formation as the research target, toregulate the microenvironment of the targets is of great significance for preventionand treatment of periodontal disease.Oligonucleotide (oligodcoxynudeotides, ODN) is a polynucleotide chain meanscontaining less than50nucleotide monomer. It is the nucleotide base sequence tounmethylated DNA sequence. ODN exists in genome DNA of certain lowerorganisms in the nature,and they can simulate the immunostimulatory effects ofbacterial DNA, activating the body types of immune cells to produce proinflammatorycytokines and other inflammatory chemical mediators involved in the body’simmunity against infection. ODN with high efficiency and low toxicity, the propertiesof stability and ease of synthesis characteristic, may be the form of areceptor-mediated cellular uptake and be able to enter the cells without further constructed vector.Thus it has become the hotspot in the field of medicalimmunology.Methods: We choose murine macrophage RAW264.7as the objective cells,thenrevived,cultured and subcultured in conventional method.And using a certain numberof vaccination in pore plate of6. After waiting for12hours, the cells are completelyadherent, we change the liquid with serum-free culture of hunger for24hours.Adopt Real-time PCR to detect the mRNA expression of NLRP3﹑IL-18﹑IL-1β﹑Caspase-1after cultivating the target cell RAW264.7with Pg, MT01, Pg+MT01, PBSrespectively for6hours.Result: Compared with PBS group, the NLRP3mRNA expression wassignificantly up-regulated by its culture with Porphyromonas gingivalis.(P<0.01)After culturing with MT01, the value of the expression became the lowest among allthe testing groups (P<0.05). However, after being cultured with Pg and MT01together, the expression value was less up-regulated than culturing with Pg only. Andit was more up-regulated than culturing with MT01only (P<0.01). And the mRNAexpression of other related factors in group Pg and MT01together are lower than thePBS group(P<0.01).Conclusion: MT01can inhibit mRNA expression of NLRP3and related factorsafter its infection with Pg.