Basic Research On The Injury Of Brains About Fluoroacetamide Poisoning Rats And The Medication

BackgroundFluoroacetamide poisoning is efficient suction action within the class of organic fluorinevirulent rodenticide, all have high toxicity for people and livestock, and has thecharacteristics of the secondary poisoning.Oral poisoning death rate is high, and the latecause toxic encephalopathy, the high incidence of legacy minimally, light person acutepoisoning symptoms of dizziness, headache, limb numbness, twitch, the person that weighcan cause multiple organ function damage, heart damage in brain, the most common, butbecause of fatal arrhythmia, systemic ankylosing clonus lead to respiratory depression anddeath.Poisoning mechanism is not yet clear, currently accepted fluoroacetamide poisoningmechanisms for blocking the Krebs cycle, cause a series of pathological changes, there is noresult in brain damage after the poisoning of further research.By discussing furtherfluoroacetamide poisoning mechanism of brain damage and effective treatment methods toreduce the case fatality rate, reduce the occurrence of toxic encephalopathy, improveprognosis.ObjectiveThe main purpose is to investigate the pathogenic mechanism of fluoroacetamide andthe relationship between oxidative damage and apoptosis,observing the treatment ofalprostadil injection in poisoning rat brain damage effect.MethodsThrough lavage methods make fluoroacetamide poisoning rats model, the random isdivided into5groups, group A as the normal control group,1ml saline lavage, dailyintraperitoneal injection of saline solution.Group B for fluoroacetamide infected group,fluorine acetamide solution according to6mg/kg to fill the stomach to producefluoroacetamide poisoning model, and a day to give isodose intraperitoneal injection ofsaline solution.For acetamide treatment group, group C fluoroacetamide poisoning after1hour in2.8g/kg for acetamide, intraperitoneal injection of twice a day.Group D foracetamide and alprostadil injection treatment group, fluoroacetamide poisoning after1hour in2.8g/kg for acetamide, intraperitoneal injection of twice a day, at the same time accordingto add alprostadil injection10ug/kg to injection, intraperitoneal injection of once aday.Group E to alprostadil injection treatment group, after1hour of fluoroacetamidepoisoning by intraperitoneal injection of10ug/kg to lead to injection, a day.In healthy ratsinfected after different time points (4h,1d,3d,7d) to Nepal’s the brain hippocampus tissuestaining and immunohistochemical staining method to observe the morphology ofhippocampal organization change and Caspase3protein expression, determination of braintissue at the same time the superoxide dismutase (SOD), malondialdehyde (MDA) content,statistical analysis, to explore its significance.ResultsThe results of Nissl’s stain mainly in neurons form change, nucleolus disappeared, ablue plaque or granular nissl body disappear, in4h after injury, severe poisoning with thepassage of time, the damage symptoms gradually decreased;Front row, joint acetamideinjection treatment group significantly reduce brain damage.Through determination of MDA,SOD content in brain tissue and Caspase3protein immunohistochemical staining andmeasured the average optical density value line statistical analysis found that rats afterpoisoning can cause higher MDA content, SOD content reduce, Caspase3increased proteinexpression.Group D injection treatment group and control group A and group B infectedgroup, the group C acetamide group comparison,the MDA content is higher than that ofgroup A, lower than that of group B, group C;SOD content is lower than that of group A,higher than that of group B, group C;Caspase3protein expression was higher than group A,lower than that of group B and group C.ConclusionFluoroacetamide poisoning can cause brain tissue damage, Caspase3protein in thefluoroacetamide poisoning rats of brain tissue had expressed, induce cell apoptosis may beone of the reasons for poisoning afterbrain tissue damage.MDA content in fluoroacetamidepoisoning rats content increased significantly, SOD content decreased significantly, considerfluoroacetamide poisoning cause brain injury related to oxidative damage.In the process ofthe treatment of fluoroacetamide poisoning, the alprostadil injection can inhibit theexpression of Caspase3protein, reduce the generation of MDA, protect the SODactivity.Therefore,the alprostadil injection has protective effect on fluoroacetamide braindamage.