The Relationship Between The Expression Of MicroRNA-150with P21and P27in Tissues Of Colorectal Carcinomas And Its Effect On Cell Proliferation

Objective:Colorectal cancer is reported as one of the most common cancers. But, itspathogenesis is not fully clear. The development of colorectal cancer is elatedto a variety of factors. miRNA play an important role in cancer. miRNAs aredown-regulated or up-regulated in various cancer types, triggering abnormalcell differentiation, proliferation and apoptosis. To examine the expressions ofmicroRNA-150(miR-150), p21and p27in colorectal carcinoma and normalmucosa using by real time PCR and study the relationship between theexpressions and the clinicopathological characteristics of patients. Then toobserve the effect of miR-150on proliferation and of colon cell lines byCCK-8assay. To provide theoretical basis for pathogenesis of colorectalcarcinoma.Methods:1Thirty-five cases of fresh colorectal tissue specimens from HebeiMedical University Fourth Hospital and Hebei Medical University FirstHospital between September,2012and September,2013were collected,including colorectal carcinoma and normal mucosa tissue.2Cell culture was performed for colon cell lines HCT116.3The expressions of miR-150, p21and p27were examined in colorectalcarcinoma and normal mucosa using by real time PCR, and study therelationship between the expressions and the clinicopathologicalcharacteristics of patients.4MiR-150minic was transfected into colon cell lines HCT116,paralleled with blank and control groups. 5CCK-8assay were performed in blank, control and experimentalgroups, OD value were detected respectively after1,2,3,4and5d oftransfection and then cell growth curve were drew.6All experimental data were analyzed by SPSS13.0statistical software.The results were measured by median (quartile). Wilcoxon-test andSpearman’s rank correlation was used to analyze comparison of paired sample.P<0.05was regarded as statistical significance.Results:1The expressions of miR-150in colorectal carcinoma tissue and matchednormal mucosa tissue were0.071(0.031,0.129) and0.336(0.168,0.817)respectively. The miR-150expression in colorectal carcinoma was lower thanthat in normal mucosa tissue, there were significant differences (P<0.05).2The expressions of p21in colorectal carcinoma tissue and matchednormal mucosa tissue were0.007(0.003,0.083) and0.036(0.012,0.164)respectively. The p21expression in colorectal carcinoma was lower than thatin normal mucosa tissue, there were significant differences (P=0.013).3The expressions of p27in colorectal carcinoma tissue and matchednormal mucosa tissue were0.016(0.001,0.048) and0.023(0.002,0.063)respectively. The p27expression in colorectal carcinoma was lower than thatin normal mucosa tissue, there were no significant differences (P=0.340).4There was correlation between expression of miR-150and p21(rs=0.434, P=0.009)；there was no correlation between expression of miR-150and p27(rs=0.033, P=0.855)5The expressions of miR-150, p21and p27were not correlated with theclinicopathological characteristics of patients. There were no differences(P>0.05).6After1,2,3,4and5d of transfection, the OD value by CCK-8assaydid not show significant difference between blank group and control group inHCT116cell lines；the OD value in experimental group were higher than thatin control group at every interval of24hours, there were significantlydifferences (P<0.0001). Conclusion:1The expression of miR-150was at a low level in colorectal carcinoma.miR-150was a potent tumor suppressor in colorectal carcinoma.2The expression of p21was at a low level in colorectal carcinoma. p21was a potent tumor suppressor in colorectal carcinoma.3MiR-150through suppresses cell proliferation to play an important roleas anti-oncogenes in the pathogenesis of colorectal carcinoma.4There may be a positive regulation cycle between miR-150and p21.They together inhibited cell proliferation.