Breast Cancer Axillary Lymph Expression And Clinical Significance Of CD83, CD86’s

Objective: lymph nodes (lymph nodes, LN) as the body’s importantimmune organ, is the body ’s main anti-tumor sites, but also an importanttumor site through lymphatic metastasis. Cancer tumor cells received fromthe sentinel lymph node tumor tissue, tumor cell debris, a tumor antigen andphagocytic antigen presenting cells (antigen presentingcells, APC) and othersubstances, to provide antigen-specific immune cells sensitized to identifyand place, and are considered to have anti-tumor immunity. But about thestudy showed that the sentinel lymph node metastasis does not comparesentinel lymph node metastasis, and its anti-tumor immune function decline,leading to tumor escape, tumor progression. Breast cancer is a malignanttumor occurred in the breast tissue, lymphatic metastasis important shift theirway, the draining lymph nodes, especially the ability to study the anti-tumorimmune sentinel lymph node, is one of the hot research findings to guidebreast cancer treatment and prognostic significance.Dendritic cells (dendrilic cell, DC) is the body ’s most powerful APC, hasa unique and efficient antigen presentation, inspire action, a bridge pivotalrole in the interaction between tumor cells and T lymphocytes in. Containsmore tumor antigens have been ingested DC, on the one hand, as a potentialsource of lymphocytes obtained on tumor cells has a strong killing effect; onthe other hand, DC also has the function of immune tolerance may be tumorand lymph node metastasis cellular immune escape. Therefore, the state ofDC ’s immune function may reflect the immune function of lymph nodecancer, the current research on cancer draining lymph nodes showed thestrength of the lymph nodes in the DC immune function primarily to itsmaturity and ability to stimulate T cell related. Has confirmed gastricnasopharyngeal study fell sentinel lymph node metastasis DC maturity, decreased antigen presentation while stimulation of T cells into cytotoxic Tcells (Cytotoxic T cell, CTL) reduced ability to cause the anti-turn tumorimmunity decline; hand, DC infiltrating the tumor tissue was positivelycorrelated with the prognosis.This study was collected from a sentinel lymph node in breast cancerclinical research material, the application of flow cytometry to detect breastcancer sentinel lymph node CD83, CD86marker expression rate, thereference node clinicopathological parameters derived relations, the use of theapplication SPSS19.0statistical package data processing, combined withprevious findings, discuss changes in immune function in breast cancersentinel lymph node status functional changes caused by the DC.Methods: July2013to December2013newly diagnosed stage Ⅰ~ⅡFourth Hospital of Hebei Medical University Breast Center treated with breastcancer and underwent surgery in patients with a total of53, the linemastectomy+sentinel lymph node biopsy in22cases row modified radicalmastectomy31patients, all patients were first sentinel lymph node removal inthe sentinel lymph node surgery, each one drawn along the lymph nodes bythe lymphatic diameter cut the door in the middle, half calf serum instantlyinto cultured, and the other half the normal inspection by the department ofPathology, formalin-fixed after. Postoperative paraffin pathologicallyconfirmed23cases without lymph node metastasis (43%),30cases withlymph node metastasis (56%). Axillary lymph node metastasis in patientswith a mean age of51years old, no axillary lymph node metastasis in patientswith a mean age of47, no statistical significance between the two ages (P>0.05). The network nodes taken legal twist into a single cell suspension,double immunofluorescent labeling CD83, CD86, flow cytometry analysis, theobserved positive and double-labeled single-labeled positive sentinel lymphnode metastasis in the state and the expression of metastasis after usingSPSS19.0statistical package for data processing and analysis, followed bybreast cancer metastasis in sentinel lymph node metastasis status and immunefunction in tumor cases. Results:1CD83, CD86double-positive expression rate in the absence of metastaticlymph nodes was6.20±4.08%, in the metastatic lymph nodes was1.14±0.48%, non-sentinel lymph node metastasis CD83, CD86expression wassignificantly higher than the double transfer group, two groups wasstatistically significant (P=0.000).2CD83expression in the absence of a single positive lymph node metastasiswas15.47±15.12%, in the metastatic lymph nodes was3.98±1.72%, non-sentinel lymph node metastasis was significantly higher than CD83expressionmetastasis group, the two groups was statistically significant (P=0.002).3CD86expression in the absence of a single positive lymph node metastasiswas12.35±12.14%, in the metastatic lymph nodes was3.69±1.76%, non-sentinel lymph node metastasis was significantly higher than CD86expressionmetastasis group, the two groups was statistically significant (P=0.00).4CD83, CD86double-positive expression rate in the tumor diameter≤2cmwas5.76±4.86%, tumor diameter>2cm was when2.39±2.70%, CD83,CD86double-positive rate of large tumor diameter was significantly lower inboth groups was statistically significant (P=0.016).5CD83, CD86double-positive expression rates of ER+and/or PR+was3.45±3.37%, in the ER (-) PR (-) was4.01±4.59%. ER+and/or PR+ofCD83, CD86double-positive rate of ER (-) PR (-) showed no statisticalsignificance (P=0.745).6CD83, CD86double-positive expression rate of Her-2positive was1.62±1.46%, Her-2negative was4.21±4.19%, Her-2positive for CD83, CD86double-positive rate was significantly lower than the Her-2negative, twogroup was statistically significant (P=0.010).7CD83, CD86double-positive expression rates have vascular invasion was2.64±3.97%, no vascular invasion was4.05±3.19%. No vascular invasion ofCD83, CD86expression was significantly lower than vascular invasion, thetwo groups was statistically significant (P=0.000).8The Institute for Organization classification into some grade Ⅱ, Ⅲgrade, CD83, CD86double-positive expression rate in tissue differentiation grade Ⅱwas3.47±3.40%, in the differentiation grade Ⅲ was3.11±3.95%, ⅡgradeCD83differentiation, CD86expression and differentiation of double positivestage Ⅲ showed no statistical significance (P=0.229).Conclusion:1In this study, breast cancer sentinel lymph node CD83, CD86double-labeled positive rate of sentinel lymph node metastasis in sentinellymph node metastasis is significantly greater. Accordingly the results,considering the sentinel lymph node in breast cancer, and reduce the numberof mature DC, reduce ability to stimulate T cell activation, the presence ofimmunosuppression.2CD83single positive rate of sentinel lymph node metastasis in sentinellymph node metastasis is significantly lower than those without. Consider thesentinel lymph node in breast cancer metastasis, DC maturation inhibition, sothe number of mature DC reduce, reduce the degree of maturity.3CD86single positive rate of sentinel lymph node metastasis in sentinellymph node metastasis is significantly lower than those without. Inconsideration of the sentinel lymph node metastasis in breast cancer, theantigen presenting process to provide costimulatory signals diminishedcapacity, the ability to stimulate the proliferation and differentiation of T cellsis reduced, decreased ability to induce CTL response, cannot effectivelyactivate T cells to kill the target decline.4Combined with postoperative paraffin pathology, tumor diameter≤2cm,CD83, CD86double-positive rate is significantly higher than the standarddiameter>2cm, explain the lower DC maturity in the sentinel lymph nodetumor diameter is large, the fewer stimulate T cell activation is weak, weakerability to induce CTL responses, so the overall anti-tumor immunity lymphnodes received suppression.5Her-2negative for CD83, CD86double-positive rate is significantly higherthan the Her-2positive, indicating higher maturity of the DC Her-2-negativesentinel lymph nodes, when the number goes greater, anti-tumor immunity be stronger, when the number of Her-2goes lower the sentinel lymph node-positive DC maturity. the fewer the number, the more likely the transfer tooccur.6No vascular invasion of CD83, CD86double-positive expression issignificantly higher than had vascular invasion, the higher the degree of DCmaturation within no vascular invasion, when the number goes greater, thestronger the anti-tumor immunity, sentinel lymph vessel invasion lower DCmaturity, the fewer the number, the more likely the transfer to occur.