The Expression And Significant Of PPAR-γand PTEN In Bladder Transitional Cell Carcinoma

Objective： To study the expression of PPAR-γ and PTEN protein in bladdertransitional cell carcinoma and the relationship between clinical/pathological parametersand the expression of PPAR-γ and PTEN protein, as well as the relationship between theexpression of PPAR-γ and that of PTEN protein in bladder transitional cell carcinoma.Methods: The expression of PPAR-γ and PTEN protein of94cases of bladdertransitional cell carcinoma confirmed by pathology and20cases of normal bladder tissuewas detected by immunohistochemistry. The expression of PPAR-γ and PTEN protein wererepresented by gray value measured by pathological analyzer. The expression of PPAR-γand PTEN protein between bladder transitional cell carcinoma and normal bladder tissuewere compared. The relationship between the expression of PPAR-γ/PTEN protein andsex, age, tumor size, clinical stage, pathologic grade, lymph node metastasis,single/multiple tumor were analysed. And the analysis of the relationship between theexpression of PPAR-γ and PTEN protein in bladder transitional cell carcinoma wereperformed.Results:①The expression of PPAR-γ protein was positive in4cases (4/20,20%) of normal bladder tissue and64cases (64/94,68%) of bladder transitional cellcarcinoma tissue. The positive rate of PPAR-γ protein between normal bladder tissue andbladder transitional cell carcinoma tissue showed a significant difference (P <0.001). Themean gray value of PPAR-γ protein expression of normal bladder tissue (189.55±6.85)was higher than that of bladder transitional cell carcinoma (151.24±9.28)(P=0.002).②The expression of PTEN protein was positive in20cases (20/20,100%) of normalbladder tissue and49cases (49/94,52.1%) of bladder transitional cell carcinoma tissue.The positive rate of PTEN protein between normal bladder tissue and bladder transitionalcell carcinoma tissue showed a significant difference (P <0.001). The mean gray value ofPTEN protein expression of normal bladder tissue (133.00±7.69) was lower than that of bladder transitional cell carcinoma (167.43±8.83)(P=0.001).③The mean gray valueof PPAR-γ protein expression of bladder transitional cell carcinoma without muscleinfiltration (162.26±9.40) was significant higher than that of bladder transitional cellcarcinoma with muscle infiltration (145.30±6.98)(P<0.001), and there was a negativecorrelation between the expression of PPAR-γ protein and tumor muscle infiltration (r=-0.688, P <0.001). The mean gray value of PPAR-γ protein expression of low-gradebladder transitional cell carcinoma (159.22±10.85) was significant higher than that ofbladder high-grade transitional cell carcinoma (151.40±11.94)(P=0.002), and there was anegative correlation between the expression of PPAR-γ protein and tumor pathologicalgrade (r=-0.321, P <0.002). The mean gray value of PPAR-γ protein expression ofbladder transitional cell carcinoma without lymph node metastasis (159.54±10.31) wassignificant higher than that of bladder transitional cell carcinoma with lymph nodemetastasis (149.74±12.15)(P <0.001), and there was a negative correlation between theexpression of PPAR-γ protein and tumor pathological grade (r=-0.391, P <0.001). Theexpression of PPAR-γ protein did not showed significant differences between eachgroups of gender, age, tumor size.④The mean gray value of PNET protein expressionof bladder transitional cell carcinoma without muscle infiltration (150.23±12.56) wassignificant lower than that of bladder transitional cell carcinoma with muscle infiltration(170.61±8.88)(P<0.001), and there was a positive correlation between the expression ofPNET protein and tumor muscle infiltration (r=0.653, P <0.001). The mean gray value ofPNET protein expression of low-grade bladder transitional cell carcinoma (153.51±16.25)was significant lower than that of bladder high-grade transitional cell carcinoma(163.91±9.67)(P=0.001), and there was a positive correlation between the expression ofPNET protein and tumor pathological grade (r=0.338, P <0.001). The mean gray value ofPNET protein expression of bladder transitional cell carcinoma without lymph nodemetastasis (153.90±15.63) was significant higher than that of bladder transitional cellcarcinoma with lymph node metastasis (164.45±10.66)(P <0.001), and there was anegative correlation between the expression of PNET protein and tumor pathological grade(r=0.333, P=0.001). The expression of PNET protein did not showed significantdifferences between each groups of gender, age, tumor size.⑤The expression of PPAR-γprotein showed a negative correlation with the expression of PNET protein in bladdertransitional cell carcinoma (r=-0.604, P <0.001). Conclusion:①The expression of PPAR-γ protein in bladder transitional cellcarcinoma is significantly higher than in normal bladder tissue, whereas the expression ofPTEN protein is significantly lower than normal bladder tissue.②The expression ofPPAR-γ protein and PTEN protein show significant correlation with clinical stage,pathological grade, lymph node metastasis.③In bladder transitional cell carcinoma, theexpression of PPAR-γ protein showed a negative correlation with the expression of PNETprotein, which suggests between these two proteins may exist a regulation requiring furtherstudy.