Protective Effects Of Levosimendan On Oxidative Damage In Aat Cardiomyocutes Induced By H₂O₂

Objectives1. To investigate whether there is effect of proliferation forlevosimendan on rat cardiomyocytes.2. To study whether there is protective effect of levosimendanagainst H2O2-induced oxidative damage in rat cardiomyocytes.Methods1. The different survival rates of rat cardiomyocytes correspondingdifferent concentrations of levosimendan were studied by MTT.2. Observe rat cardiomyocytes morphology when treated bylevosimendan and H2O2with light microscope.3. The rat cardiomyocytes apoptosis rate was examined by Flowcytometer technology when treated with levosimendan and H2O2.Results1. Results of MTT:①Normal rat myocardial cells were treated bydifferent concentrations of levosimendan from0.03to0.24umol/L.The result of MTT showed that there is no significantdifference between the treated group and normal control group.②When treated by levosimendan which concentration was higher than0.3umol/L the optical density was much lower than that of normalcontrols.③The optical density fell obviously after treated byH2O2..④The optical density of the group which pretreated bylevosimendan did not fall as obviously as that was withoutpretreating. The optical density changed with the concentrationof levosimendan.2. The cell morphology were observed by light microscope:①The ratcardiomyocytes which were treated by levosimendan only showedfusiform cells, radial or fence-like arrangement. Morphology ofrat cardiomyocytes did not change with different concentrationsof levosimendan.②The number of rounded cell bodies of thegroup treated by H2O2which concentration was1000umol/L were much more that was500umol/L.③The levosimendan could protectecardiomyocytes from H2O2, there were more rounded cell bodies thancontrol group with higher concentration of levosimendan, thehigher the concentration of levosimendan, the less shrinkage ofcell pseudopods..3.Results of Flow cytometer:①The control group result is that theearly apoptosis rate, late apoptosis rate,necrotic rate and thesurvival rate were0.31%,6.88%,4.65%and88.16%respectively.②The H2O21000umol/L group result is that the early apoptosis rate,late apoptosis rate,necrotic rate and the survival rate were1.52%,8.03%,7.66%and82.79%respectively.③The Levo0.24umol/L+H2O21000umol/L group result is that the early apoptosis rate, lateapoptosis rate,necrotic rate and the survival rate were0.44%,6.48%,4.95%and88.13%respectively.Conclusions:1.Levosimendan has no proliferation on myocardial cells of normalrats.High concentrations of Levosimendan has inhibitory effectson the proliferation.2.Levosimendan has protective effects against H2O2-induced oxidativedamage in rat cardiomyocytes. The protective effect is much moreobvious when the concentration of Levosimendan is0.24umol/L thanthat of0.03umol/L.