Study Of Immunogenicity And Anti-thrombotic Effectt Of Tenebrio Fibrinolyric Proteins

Tenebrio molitor also called mealworms,under Coleoptera,Tenebrionidae,Mealworm genus.It is native to North America and it was introduced to ourcountry in the1950s.Tenebrio molitor is rich in a variety of nutrients and its dry goods contains protein up to50%above.So it is known as treasury of protein feed.Moreover,Tenebrio molitor is rich in phosphorus,potassium,iron,sodium,aluminum and other mojor elements and a variety of trace elements and16kinds of amino acids. Adds on the advantages of strong survivability and adaptiveness, quick growth, low cultivation cost, Tenebrio molitor had already become the third fastest growing worm-raising industry in the world only after silkworms-raising and beekeeping.With the continuous improvement of people’s living standard in our country,health and longevity has increasingly became the urgent needs of the people.Research shows that the thrombotic disease is one of the most serious disease that threatens human health in recent years. It has a very high morbidity and mortality rate and its morbidity and mortality rate continues to rise.Therefore,effective prevention and treatment thrombosis has a very important significance of protecting the public health and improving the quality of human life.In recent years,we extracted a potent thrombolytic activity protein from Tenebrio molitor.This study expects to detecting a series of physical and chemical properties and imm unogenicity of the active protein,to make the basis that it can be used as an effective and safe therapeuticA novel of fibrinolytic protein named Fibrinolyric Tenebrio Proteins (TFP), was separated and purified from Tenebrio molitor by the methods of homogenate leaching mention, salting and anion exchange method. Fiber tablet method showed TFP had good fibrinolytic activity;Bradfore method showed protein concentration was7.7905mg-mL-1; SDS-PEAG showed The relative molecular weight was about54.3kD.To study the immunogenicity TFP.Mice were immunized purified TFP by subcutaneous injection,then the antibody content and antibody titer were detected by the optimal ELISA method. The results suggested that the TFP has the similar immunogenicity with urokinase. The specific antibody titer was to be1:640after the last immunization.The antithrombotic experiments in vivo indicated the TFP could inhibit the thrombus forming on mice tail induced by carrageenan,delay thrombin time(TT) in mice obviously,enhance tissue plasminogen activator(t-PA) activity and inhibit plasminogen activator inhibitor(PAI) activity and inhibit plasminogen activator inhibitor activity significantly.The results suggest that TFP could suppress thrombosis.