Macrophage Depleted Uranium Particles And The Supernatant Particles Affect Phagocytosis Of Macrophages In Bronchial Epithelial Cells

ObjectiveThe aim of this study was to simμlate macrophages in human lung phagocyticdepleted uranium particles,on macrophage injury and the supernatant particles affectphagocytosis of macrophages in bronchial epithelial cells.Methods:In macrophages (AM), using insoluble depleted uranium exposure onmacrophages,by transmission electron microscopes to observe the way of phagocyticdepleted uranium particles. Determination of phagocytic process of acid phosphatase(ACP), lysozyme (LZP) changes by enzyme-linked immunosorbent assay.Determination of uranium particles after phagocytosis of soluble uranium content in thesupernatant of macrophages by wet digestion method. Chips were measured by a liquidsuspension in the supernatant was measured IL-1β、IL-10、IL-6、GM-CSF、IL-5、IFN-γ、TNF-α、IL-2、IL-4、 IL-8, etc. cytokine levels. Determination of macrophage survivalby CCK-8assay. Chemistry/fluorescence emission detected in different concentrationsof depleted uranium on macrophage ROS changes. Using the Griess Reagent method,the DTNB assay living cells reactive nitrogen (NO) and glutathione changes.Human bronchial epithelial cells (BEP-2D) for the study, Determination ofBEP-2D survival by CCK-8assay. Chemistry/fluorescence emission detected indifferent concentrations of depleted uranium on BEP-2D ROS changes. Results:Transmission electron microscope, DU particles eventually engulfed in lysosomesdigest, and with increasing particle concentration, DU particles into macrophages in thevacuole.ACP increased with time and concentration levels of the enzyme is decreased,and the content does not appear LZP rise with the increase or decrease in theconcentration and time. Supernatants of macrophages increased significantly solubleuranium. IL-1β, TNF-α, IL-4, IL-8increase the expression of cytokines.Significantlydecreased macrophage viability.macrophage intracellular reactive oxygen species,reactive nitrogen increased glutathione decreased.supernatant after macrophagephagocytosis of DU particles makes BEP-2D cell viability decreased, ROS rose.Conclusion:DU macrophages after phagocytosis was dissolved digested in the lysosomes to asoluble uranium, and the mechanism of oxidative stress injury bymacrophages.macrophages stimulated by DU supernatant applied to the BEP-2D, thesupernatant produce cytokines and oxidative damage plays a key role in survival.Graduate student: SunpingDirected by Professor ZhumaoxiangDept of Public Health, Soochow University Suzhou...