The Expression And Significance Of SOCS-3and Bcl-2/Bax、caspase-3in Endometriosis

BackgroundEndometriosis is one of benign gynecological diseases in women of reproductive age. It affects10-15%women, especially as high as20-40%in infertile women. Because of its etiology and pathogenesis not yet being fully interpreted, it’s hard to diagnosis and treatment. Much research has recently focused on the association of endometriosis with apoptosis inhibitation. While endometriosis is a benign disease, it still has some kind of characteristics like tumor metastasis and invasion. We choose SOCS (expressed in many tumors as silence signal)-3, Caspase-3(a key prolease in apoptosis) and Bcl-2/Bax (important regulatory factor in cell mitochondrion apoptosis pathway) to investigate those factors expressions in ectopia endometrium and in situ endometrium, and to study the effects on endometriosis pathogenesis for the sake of finding new targets of treatment.ObjectiveTo search the expressions and correlation among socs-3,Bc1-2/Bax and Caspase-3in endometriosis, and to further understand the pathogenesis of endometriosis. MethodsUsing two-step immunohistochemisty to detect SOCS-3and Bcl-2/Bax, Caspase-3protein expression in ectopic and eutopic endometrium (n=32) of patients with endometriosis, as well as normal endometrium (n=30) of women absence of endometriosis.Results1.the SOCS-3、Bcl-2/Bax and Caspase-3were expressed in different degrees in the eutopic endometria and the ectopic endometria in Ems patients and normal endometrium in control patients. The expression was mainly in the glandular epithelium and cavity epithelium.2. The expression of SOCS-3was not influenced by the menstrual cycle (P>0.05). but the expression of SOCS-3in ectopic endometrium was significantly lower than that in eutopic (P=0.001) and control endometria (P<0.001). SOCS-3expression in the ectopic and eutopic endometria were obviously lower in phase of Ⅲ/Ⅳ than that in Ⅰ/Ⅱ of endometriosis.3. Menstrual cycle and staging of endmetriosis have no effect on caspase-3expressions in three endometrium groups(P>0.05), while Significantly lower expression of Caspase-3protein was found in ectopic and eutopic endometria when compared with the control endometrium (P<0.001).4. Bax expression demonstrates periodical changes in control endometrium:secretory phase higher than proliferative phase (p<0.05),while no perodical changes in ectopic and eutopic endometrium in endometriosis (p>0.05), and either secretory phase or proliferative phase in ectopic endometrium lower than those in in eutopic endometrium (p<0.05) and control (p<0.001). Bcl-2expression also shows periodical changes: proliferative phase higher than secretory phase (p<0.001). While in endometriosis, bcl-2doesn’t have periodical changes (p>0.05). Bcl-2expression in ectopic endometrium of secretory phase or proliferative phase is significantly higher than those in in eutopic endometrium and control (p<0.001).5. In ectopic endometrium, there is no correlation among SOCS-3、Bcl-2and Bax, while SOCS-3has positive correlation with Caspase-3(r=0.655)Conclusion1. Bcl-2shows higher expression and Bax lower expression in ectopia endometrium, while Caspase-3displays lower expression in ectopia and eutopia endometrium. The results indicate the ability of apoptosis decreases in ectopia and eutopia endometrium, which maybe involve in the process of endometriosis pathogenesis.2. SOCS-3demonstrates lower expression in ectopia endometrium. With the progression of the disease, SOCS-3decreases aggressively, which maybe indicate SOCS-3has connection with staging of endometriosis.3. SOCS-3induces the progression of endometriosis by inhibiting the endometrium cell apoptosis.