The Effect Of Alternate Day Fasting To Serum And Liver SOD, GSH-PX Activity And MDA Content In NAFLD Rats

Objective:Non-alcoholic fatty liver disease(NAFLD) is a diseases relatewith genetic, environment and metabolism.One of the main pathogenesis isthe two hit theory which the main is oxidative stress. The obesity significantlyis increasing because the improvement of living standard, the change of dietstructure and the lack of physical exercise. On nutrition and energymetabolism, the main reason of fatty liver is excess energy is stored in yourbody by too much high calorie food intaked and physical exercise decreased.At present, there is no specific drug in the treatment of NAFLD, Lose weightby lifestyle changing is a based treatment for Obesity patients withnon-alcoholic fatty liver. The studys found, only diet regulation on NAFLDpatients can reduce weight, serum triglyceride and cholesterol, elevate highdensity lipoprotein, insulin sensitivity, reduce liver triglyceride. Fastingtherapy is stop intake all food except water, some vegetable or fruit juice for alimited period of time, it is the ability to meet the body’s requirements bymaking use of the body’s energy reserves, to achieve treating or preventing ofdiseases.Studies show that, fasting therapy can slow down the energymetabolism, reduce weight, regulate the body’s metabolism, and it is provedthat can delay aging,treat and prevent the chronic degenerative diseases andthe chronic inflammatory disease. The purpose of this study is to investigatethe effect of alternate day fasting to serum and liver MDA, SOD, GSH-PX.Observe the treatment effect and possible mechanism in NAFLD rats, so as toprovide experimental basis for human.Methods:Fifty male wistar rats were randomly divided into2: groupⅠisBlank group(n=12), fed with normal food,groupⅡis NAFLD model group(n=38), fed with high-fat food. Eight weeks later, random selecting2rats fromeach group and mading liver pathological sections. After Confirming theNAFLD model was successful established, groupⅠ(n=10), continue fed withnormal food, the remaining groupⅡrats were divided into4groups randomly, 9rats in each group: group A is control group, fed with normal diet, B isalternate day fasting (ADF) groupe, group C is caloric restriction (CR)group,group D is NAFLD group. End of the12week, take serum and livertissue specimens, make liver homogenate and pathological sections. Testingthe content of MDA, the activity of SOD and GSH-PX in serum and livertissues. Data analysis was carried out with the SPSS17.0statistical softwarepackage. Measurement data to meet the normal distribution andhomogeneityof variance. The comparison processing was used single factor varianceanalysis (one-way ANOVA), group two two compared with SNK method.Results were used the mean±standard deviationg(x±s) representation. P<0.05,the difference was considered significant.Results:①8weeks high fat diet lead to hepatic lipid deposition in rats,NAFLD model was successful established;②Changes in body weight andhepatic index: At the end of the12week, the body weight and hepatic index ofgroup D showed more significantly increase than groupⅠ(P<0.05), comparedwith group D, the body weight and hepatic index of group B and group Cshowed significantly decrease(P<0.05), body weight in the B group was lowerthan group C (P<0.05), but there was no significant difference between thetwo groups of liver index(P>0.05);③Changes of liver pathology: Liver cellswelled and arranged disorderly, there were a lot of lipid droplet andInflammatory cells in cytoplasm in group D, the cells were uniform andarranged orderly, a few lipid droplet in cytoplasm in group B and group C,liver cell swelled and arranged disorderly, there were a lot of lipid droplet incytoplasm in group A, but were lesser than D;④The level of serum MDA,SOD, GSH-PX in various groups: At the end of the12week, compared withgroupⅠ, the content of serum MDA showed significantly increase (P<0.05),and the activity of serum SOD and GSH-PX showed significantly decrease ingroup D (P<0.05), compared with group D, the content of serum MDAshowed significantly decrease (P<0.05), and the activity of serum SOD andGSH-PX showed significantly increase in group B and group C (P<0.05), thelevel of serum MDA, SOD, GSH-PX was no significant difference between group B and group C(P>0.05);⑤The level of liver tissue MDA, SOD,GSH-PX in various groups: At the end of the12week, the content of liverMDA showed significantly increase (P<0.05), and the activity of liver SODand GSH-PX showed significantly decrease in group D than groupⅠ(P<0.05),compared with group D, the content of liver MDA showed significantlydecrease in group B and group C (P<0.05), the activity of liver SOD andGSH-PX showed significantly increase in group B and group C (P<0.05),compared with group C, the content of liver MDA showed lower in group B(P<0.05), the activity of liver SOD showed higher in group B (P<0.05), theactivity of liver GSH-PX showed hinger in group, but it was no significantdifference P>0.05).Conclusion:1High fat diet lead to hepatic lipid deposition in rats,NAFLD modelt was successful established.2Alternate day fasting canincrease the body weight and hepatic index of NAFLD rats, lead to livertissue recovery. Alternate day fasting can increase the activity of SOD andGSH-PX, decrease the content of MDA in NAFLD rats, Alternate dayfasting can treat fatty liver by improving the ability of anti-oxidation,reduceoxidative stress;3Alternate day fasting is better than caloric restriction inreducing oxidative stress response in liver of NAFLD rats.