| Objective: Degenerative lumbar scoliosis is generally considered asspinal deformity. In coronal plane, its Cobb angle is not less than10degreeangle. It often occurs after skeletal maturity. With complex risk factors andlonger duration, this disease shows progressive trends, which is more commonin the elderly over the age of50. The number of woman with degenerativelumbar scoliosis is more than men. With aging, the incidence of this diseasehas gradually increased. The clinical symptoms of degenerative lumbarscoliosis are varied, such as low back pain, neurogenic claudication and lowerextremity neurologic symptoms of unilateral or bilateral, which lead to seriousimpact on the quality of life of patients. So, the study of degenerative lumbarscoliosis is gradually deepening. In recent years, the factors related toformation and development of degenerative lumbar scoliosis have beenmore and more researched. One point of view is that the progress ofdegenerative lumbar scoliosis is associated with paraspinal muscledegeneration. Another theory is that the etiology of degenerative lumbarscoliosis are asymmetry degenerative of disc and facet and the primary factoris the disc degeneration. But the specific mechanism remains unclear. In thepreparation of an animal model of idiopathic scoliosis domestic and foreignscholars have made many attempts and there is no mature degenerative lumbarscoliosis. So, we want to develop a scientific and economical degenerativelumbar scoliosis animal model.Methods: One-month-old female Sprague-Dawley (SD) rats30with noscoliosis were selected randomly. Firstly, by amputated upper limbs of rats andrat tail to prepare bipedal rats model. To adjust accordingly the height of foodand water based on the growth rate of the bipedal rats. After four months,bipedal rats fully adapted to walk upright and its skeletal is maturity. To acupuncture bipedal rats L4-5、L5-6、L6-S1discs one side through the methodof Rousseau. Then, the anteroposterior X-ray of the bipedal rats lumbar weretake when bipedal rats were reared to10months of age. The Cobb angle anddisc height index were obtained from degenerative lumbar scoliosis bipedalrats. The L4-5、L5-6、L6-S1discs were taken for hematoxylin and eosin (HE)staining. The morphological changes on both sides of the disc were observedunder microscope and were scored. The measured data were analyzed withtwo related sample Wilcoxon rank sum test.Results: After the first surgery7-8days，bipedal rats had been able to eatand drink rely bipedal upright posture and14days the bipedal rats had beenbasically able to walk freely, But, there is an angle between the trunk and theground in order to maintain balance of the body. Walk forward through thelower limbs. At rest the chin and abdomen were usually affixed to the ground.After the second operation, the bipedal rats recovered to the preoperative stateof motion3-4days later. There were two bipedal rats died after the firstoperation and the reason was likely to the bipedal rats was sensitive toanesthetic which leaded to overdose anesthesia preoperative. The other reasonis that the operation time was too long due to unskilled operative. After thesecond surgery,5bipedal rats died and the cause of death may be excessivesurgical trauma which caused the bipedal rats can not adapt to the dietaryconditions leading to postoperative poor diet28/30bipedal rats survived. Afterthe success of disc puncture,23bipedal rats lived, of which seven occurreddegenerative lumbar scoliosis. The average scoliosis angle was15.9°±2.3°. Both sides of the disc height index showed significantdifferences(P<0.05). The results of the both sides of the disc histological scorewas statistically significant(P<0.05).Conclusion: The animal model of degenerative lumbar scoliosis has beensuccessfully established. However, in this study only7cases appeareddegenerative lumbar scoliosis and repeatability is not enough. A large samplestudy and in-depth study of the molecular mechanisms are still needed. |
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