The Mechanism Reseacrh Of Tougu Xiaotong Capsule On Intevrention Of Osteoarthritis Cartilage Degeneration

ObjectionBy observing the TouGu XiaoTong capsule on rat osteoarthritis pathological changes of articular cartilage and the degeneration of cartilage cell autophagy in LC3I/II, ULK1, Beclinl expression, the influence of explore TouGu XiaoTong capsule on the mechanism of action of osteoarthritis cartilage degeneration.Methods44SD rats, using the method of the draw will be SD rats were randomly divided into2groups, building module33, only11normal group. Building module by papain injection preparation in the rat model of osteoarthritis, previous research has identified model preparation success [17]. Mold is to be built according to the draw method successfully build module rats were randomly divided into3groups, each group of11, respectively:TouGu XiaoTong capsule group, glucosamine group and model group.. TouGu XiaoTong capsule group with TouGu XiaoTong capsule lavage solution.. Glucosamine group of aqueous solution with glucosamine lavage, dosing1times a day, for12weeks in a row. Lavage intervention after12weeks, will kill all the animals, and left knee joint X-ray films in cartilage tissue under test, at the same time using scanning electron microscope, light microscope and transmission electron microscope, tissue morphology observation, Western blot detection cartilage LC3I/II, ULK1, Beclinl expression.Results1. The X-ray observations:Model group:the medial knee gap significantly narrowed, articular surface hardening, medial femoral condyle, tibia platform edge and edge joints have obvious osteophyte formation. TouGu XiaoTong capsule groups:knee medial narrow gap, but change the light in the model group, joint surface slight hardening, joint marginal osteophyte formation, slightly the subchondral bone density increased slightly. Compared with model group, TouGu XiaoTong capsule group, glucosamine overall morphological changes of the knee joint was obvious difference in rats.2. Cartilage Scanning electron microscopy (sem) results:normal articular cartilage surface is smooth, is typical of corrugation structure, no cartilage cell and collagen naked. Model group:cartilage collagen fiber exposed surface, formation water erosion change, enlargement of collagen fibres, fracture, peeling, circular protuberant, cartilage surface ridge structures disappear entirely, rough surface, chondrocytes fossae completely exposed and full space truss structure completely disappear. TouGu XiaoTong capsule groups:collagen fiber bare surface layer, the arrangement is irregular, some chondrocytes crypt exposed between the remnants of collagen fiber. Group compared with model group, glucosamine, TouGu XiaoTong capsule groups on the surface of the cartilage damage degree have obvious difference, the normal group compared with model group tibia platform to keep good integrity and has a smooth contour, significant differences.3. Safranin O-fast green staining:normal Groups:articular cartilage surface is smooth, the four layers of structure is clear, neat rows of cartilage cells, did not see the cartilage cells clustering,1can be divided into shallow table, transitional layer (The middle layer), deep layer (radiation), calcification, tide line complete, chromatin distribution even, clear nuclei, dyeing uniformity. Model group:articular cartilage surface rough, part of the cartilage cell nucleus atrophy, necrosis, calcified layer cartilage cell aggregates, irregular arrangement, four layers structure is not easy to distinguish, tide line is incomplete or disappear completely, even the subchondral bone exposure and capsule form. TouGu XiaoTong capsule groups: articular cartilage surface is not smooth, four layers structure can still distinguish, slightly thin cartilage layer, The middle layer cells and deep mild hyperplasia, can dye dyeing proteoglycans becomes shallow, rules, the arrangement of cartilage cell proliferation of chondrocytes and visible, in nests complete tide line. Compared with the normal group, model group cartilage has distinct histologic changes of tibial plateau. Compared with model group, TouGu XiaoTong capsule group, glucosamine tibial cartilage tissue (learning, have obvious difference. TouGu XiaoTong capsule group, glucosamine Mankin cartilage rating of group and model group were significantly higher than that of normal group (P<0.01), TouGu XiaoTong capsule group and Mankin cartilage rating of glucosamine group were significantly lower than that of model group (P<0.01).4. canning electron microscopy (sem) results:Model group compared with normal group, cartilage cells and bone cell ultrastructure of contrast difference is obvious. Compared with model group, TouGu XiaoTong capsule group, glucosamine, cartilage and subchondral bone degradation is not very serious.5. Western blot detection cartilage LC3I/II, ULK1, the expression of Beclinl:Model group LC3I/II, ULK1and Beclinl protein expression significantly lower than normal group (P<0.01). TouGu XiaoTong capsule, glucosamine LC3I/II, ULK1and Beclinl protein expression is significantly higher than model group (P<0.01).conclusions1. TouGu XiaoTong capsule can by improving the subchondral bone collagen fibers and the arrangement of calcium phosphate crystallization, inhibit the tide line copy, in order to delay the degeneration of articular cartilage.2. TouGu XiaoTong capsule can increase LC3I/II, ULK1and Beclinl protein expression level, improve the activity of cell autophagy, protect the cartilage cells.