| Abstract: Objects:The present study was designed to investigate theameliorating effect and the underlying mechanism of rTMS on vascularcognitive impairment in rat mode and provided more evidence for clinicalapplications of rTMS.Methods: The cognitive impairment mode was established via blockingfour branches of vascular which known as Pusinelli method. Magneticstimulation group and the sham-operated group were exposure to Magneticstimulation one time everyday and exposed for at least two weeks. Before andafter exposure, Water maze test was used to estimate the cognitive functionand after the two weeks therapy, rats were decollated to get samples tomeasure the expression of IGF-1,PI3K,P-Akt in CA-1hippocampus byimmunohistochemical methods.Results:(1)The results of Water maze test: After two weeks magneticstimulations, The average escape latency, the times of across cartel, theoriginal platform quadrant swimming time showed a significant difference inrat modes between stimulated and not stimulated groups(P<0.05).(2)Immunohistochemical results revealed that magnetic stimulation for twoweeks significantly up-regulated the expression of IGF-1,PI3K,p-Akt in CA-1hippocampus as compared to the s-rTMS groups(P<0.05).(3) About80adultrats get surgery in our experiment, but only31rats considered to be thesuccessful mode(45.6%,31/68),the mortality of rats in our experiment aftersurgery was about42.6%(29/68).Conclusion:(1)It had been demonstrated that rat vascular cognitive impairment model was feasible and stable through Pusinelli methods.(2)Highfrequency(10HZ) magnetic stimulation could efficiently improve the spatiallearning and memory ability.(3)The expression of IGF-1was up-regulatedafter rTMS in CA-1hippocampus and thus might be the underlyingmechanism of rTMS on improving cognitive functions.(4) Expression ofPI3K,p-Akt in CA-1hippocampus was increased in vascular cognitiveimpairment rats and this effects might mediated via IGF-1associatedsignaling pathways. |
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