The Effects Of Growth Inhibition And Apoptosis Induction Of Artemether On Tca8113Human Tongue Cancer Cells

Objective:Artemether is a derivative of artemisinin which is one of antimalarial medicines. In recent years, studies have shown that there are anti-tumor effect of artemether. In this study, it is projected to explore the effects of growth inhibition and apoptosis induction of artemether on Tca8113human tongue cancer cells and its mechanism.Methods:1Tongue cancer cells Tca8113were bought in nanjing KaiJi biotechnology limited company, used for training, experimental need.2Experimental group:divided into normal control group, different concentration artemether group. Normal control group was treated with10%FBS of1640culture medium, while artemether groups with100μg/ml,200μg/ml,300μg/ml’s artemether3MTT colorimetric assay was applied to investigate the effects of artemether on the proliferative activity of the Tca8113cells.4Flow cytometry was applied to observe the role of artemether on early apoptosis of. Tca8113cells.5Flow cytometry was applied to observe the effect of artemether on sub-diploid peak of Tca8113cells.6Immunocytochemistry method was applied to observe the effect of artemether on survivin expression in Tca8113cells.7Western-blot method was applied to examine the expression of survivin in Tca8113cells8. The statistical analysis was performed with the SPSS11.5using one-way analysis of variance (ANOVA) and LSD-t test. P<0.05and P<0.01were considered statistically significant. Result:1The impact of artemether on the value-added activity of Tca8113cells.Treated with100μg/ml,200μg/ml and300μg/ml concentration of artemether for24h,48h and72h respectively, the growth of Tca8113cells had showed time-dependent and dose-dependent manner.2The impact of Artemether on the early apoptosis of Tca8113cells (AnnexinV).100μg/ml,200μg/ml and300μg/ml concentrations of artemether treated with Tca8113cell for48h,compared with the control group, the early apoptosis rate increased gradually. While between100μg/ml and200μg/ml group, the difference was not statistically significant (P>0.05), other groups had statistically significant differences (P<0.05).3The effect of Artemether on the sub-diploid peak of Tca8113cells.100μg/ml,200μg/ml and300μg/ml concentrations of artemether tr-eated with Tca8113cells for48h, the apoptosis rate (sub-diploid peak) compared with the control group increased gradually, but control group and100μg/ml group,100μg/ml and200μg/ml group had no significant difference(P>0.05),other groups had statistically significant differences (P <0.05).4The effect of Artemether on the expression of survivin in Tca8113cells using immunocytochemistry.100μg/ml,200μg/ml,300μg/ml ART treated with Tca8113cells for48h, cell cytoplasm was observed under the microscope the brown gradually weakened and deeply colored cytoplasm of the control group, showed cytoplasmic Clear brown, through statistical analysis, the difference was statistically significant between groups (P<0.05).5Survivin protein expression resultsWestern-Blot analysis showed that:the control group and100μ/mL,200μg/mL,300μg/mL of the Artemether Tca8113cells treated with for48h, Survivin protein expression decreased progressively, through statistical analysis, the difference between the groups were statistically significant (P<0.05)Conclusion:Artemether inhibited the growth of Tca8113cells and induced the apoptosis, and its mechanism may be related to downregulation of survivin protein expression.