| Objective:Nonalcoholic fatty liver disease and chronic viral hepatitis B are common diseases which do harm to human health seriously. A large number of clinical studies have shown that the occurrence and development of liver disease are closely related to the change of gut microecology in human body.Our study aims to clarify the influence of NAFLD and CHB with NAFLD on the structure of gut microbiota by assessing the differences among the composition and quantity of the gut microbiota in patients with the diseases and in health individuals.Methods:Fresh faecal samples from47NAFLD patients,18CHB with NAFLD patients who were definitively diagnosed by pathology and34health individuals were examined to analyze the structure of gut microbiota by using bar coded454pyrosequencing technology.Results:Compared with the healthy individuals, the overall diversity of gut microbiota in NAFLD patients and CHB with NAFLD patients were lower (both p<0.01)).NAFLD patients had significant intestinal dysbacteriosis, characterized by the increasing ratio between Firmicutes and Bacteroidetes, while merging CHB may aggravated intestinal dysbacteriosis in NAFLD patients.The abundance of Firmicutes and its Erysipelotrichi class had significantly increased in NAFLD patients and CHB with NAFLD patients (all p<0.05),while the abundance of Bacteroidetes and its Bacteroidia class had significantly reduced (all p<0.05). In Genus level,different from healthy individuals, Lactococcus had significantly reduced in NAFLD patients and CHB with NAFLD patients (both p<0.01),while Streptococcus had significantly increased (both p<0.05)Conclusions:Our study suggested that there was a strong relationship between diversity of gut microbiota and NAFLD or CHB with NAFLD.The two diseases may lead to significant structural changes of gut microbiota.These changes presented in reduced bacterial diversity and alteration of some key populations. |
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