The Association Of FCRL-3Gene Single Nucleotide Polymorphisms With Graves’ Disease Of Hans In The Northern Part Of Anhui Province

Objective1.Exploring the correlation between FCRL-3gene single nucleotide polymorphismswith genetic susceptibility associated with GD in Han nationality located in thenorthern of ANHUI province by detecting allele and genotype frequency distributionsof FCRL-3gene promoter sites-169A/G single nucleotide polymorphisms (singlenucleotide polymorphisms, SNPs) in patients with diffuse toxic goiter (Graves’disease, GD) and healthy control population.2.Further investigate the correlation between FCRL-3gene promoter-169locus A/GSNPs with GD ’s clinical features, including age, sex, degree of goiter, with orwithout Graves’ ophthalmopathy, thyroid stimulating hormone receptor antibody(TRAb) level, thyroid peroxidase antibody(TpoAb), antithyroglobulin(TgAb) andthyroid function.Methods1.The experimental unrelated Han people which were born and lived in NorthernAreas of ANHUI province were divided into GD group (180cases) and controlgroup (146cases). DNA was extracted from peripheral blood accompanied with thepolymerase chain reaction-restriction fragment length polymorphism (PCR-RELP)genotype analysis was performd to compare group genotype and allele frequencies.Restriction enzyme was used to detect SNPs allele genotyping of GD patients andhealthy controls chromosome1restriction FCRL-3gene promoter sites region, thenanalyzed SNPs with GD susceptibility.2.Analyzing the association between SNPs allele genotyping and clinical indicators.Results1. The genotype frequency of FCRL-3gene promoter-169locus A/G in GD groupwas higher than control,respectively28.9%,13.8%, was statistically different between groups(χ2=6.618,P=0.046); the genotype frequency of allele G in GDgroup was higher than in control groupl,respectively49.4%,40.4%,also hasstatistically significant difference between groups(χ2=5.308,P=0.021).2. The TRAb level of GD patients was significantly different between AA, AG,GG genotype subgroups which were existed in FCRL-3gene promoter-169sites(χ2=7.319, P=0.026).But there was no statistically difference ofindicators such as sex, degree of goiter, with or without Graves’ ophthalmopathy,thyroid peroxidase antibody(TpoAb), antithyroglobulin(TgAb) and thyroidfunction((all P>0.05) between the three genotype subgroups.Conclusion1.FCRL-3gene promoter sites-169A/G single nucleotide polymorphism isassociated with the pathogenesis of GD in Han population living in the Northernpart of ANHUI province, G allele may be a susceptibility gene for GD.2. Allele G of FCRL-3gene promoter-169sites may relate to thyroid stimulatinghormone receptor antibody (TRAb),but have no concern with age, sex, degree ofgoiter, with or without Graves’ ophthalmopathy, TpoAb, TgAb and thyroid functionin GD.