Recombined IL-17a On Neutrophil Apoptosis In Rats With Severe Sepsis Caused By Actinetobacte

Objective: To study neutrophil apoptosis at different time points in the situation ofAcinetobacter baumannii sepsis in rats, and after two different doses of recombinantIL-17a therapeutic intervention, neutrophil apoptosis rate, caspase-3activityexpression of apoptosis-related proteins Bcl-2and Bax protein in the plasmaconcentration of soluble Fas were detected, and inflammatory cytokines IL-17, aswell as liver and lung tissue pathological changes were observed, the effects ofrecombinant IL-17a on abalone Man Acinetobacter neutrophil apoptosis and itspossible mechanism.Methods: Adult male Sprague1,90wistar rats were randomly divided into ninegroups: normal control group (N), Acinetobacter baumannii sepsis12h,24h group(S12h, S24h), Acinetobacter baumannii sepsis disease+saline group (SN12h, SN24h),low-dose recombinant IL-17treatment12h,24h group (SL12h, SL24h), high doses ofrecombinant IL-17treatment12h,24h group (SH12h, SH24h). Which Acinetobacterbaumannii and treatment of sepsis in each group of rats were injected Acinetobacterbaumannii suspension, intraperitoneal injection of normal saline control group;recombinant IL-17low-dose high-dose treatment for all rats were injectedintravenously rIL-17a0.001ug,1ug, I groups (Acinetobacter baumannii sepsis+salinegroup) rats were intravenously injected with normal saline.2, the normal group, thedifferent time points Acinetobacter baumannii sepsis group and rIL-17a treated ratcarotid artery blood taken after liver and lung tissue extracts of neutrophils by densitygradient centrifugation, and plasma was separated by morphology and DNAfragmentation were analyzed for apoptosis detection, the detection of changes inneutrophil apoptosis by AnnexinV-FITC and PI double staining, neutrophils Caspase3activity was detected by spectrophotometry, IL-17and soluble Fas were detected byELISA, neutrophil Bax, BCL-2protein expression were detected by western blotting,and liver and lung tissue pathological change was detected by HE staining.Results: Compared with normal control group, Acinetobacter baumannii sepsis eachgroup neutrophil apoptosis rate was significantly lower than the normal control group(P <0.05), with the other groups compared with intravenous injection of pus high-dosepoisoning rats rIL-17inhibited PMN same time point of apoptosis (P <0.05), Acinetobacter baumannii sepsis+showed no significant difference (P saline group andsepsis group>0.05);2, neutrophil DNA electrophoresis showed that high-dose IL-1712h,24h appears ladder electrophoresis patterns, other groups are in the DNAelectrophoresis bands of sample near the hole;3, with the other groups of neutrophilsCaspase3activity compared to the higher dose IL-17group12h, caspase-3activitywere significantly elevated24h (P <0.05), while the lower dose IL-17group12h,24hof caspase-3activity lowest (P <0.05), Acinetobacter baumannii sepsis and septic+saline group Caspase3no significant change in the activity (P>0.05);4, comparing thelevel of each group S-Fas, low-dose IL-17group,12h,24h highest (P <0.05),high-dose IL-17group,12h,24h lowest (P <0.05), Acinetobacter baumannii sepsis+saline group showed no significant difference with the sepsis group (P>0.05);5, HEstaining visible Acinetobacter baumannii sepsis groups of liver inflammation, celldamage was obvious, low-dose group appeared heaviest inflammation, necrosis ofliver cells, and the high-dose group compared to its relief, the normal control groupshowed no abnormalities.6, comparison with other groups, neutrophil Westernblotresult: high-dose IL-17group12h,24h of PMN maximum of BAX protein expression(P <0.05), low-dose IL-17group12h,24h least expression of BAX (P <0.05), and forprotein expression of BCL-2PMN, the high-dose group12h,24h was the lowest (P<0.05), low-dose group was the highest (P <0.05), Acinetobacter baumannii pus Therewas no significant difference (P>0.05) thyrotoxicosis+saline group and sepsisgroup.Conclusions:1. Recombinant IL-17a timely and appropriate intervention inAcinetobacter baumannii apoptotic neutrophils in septic rats may become appropriatemeasures to treat sepsis;2. higher doses of recombinant IL-17a Acinetobacterbaumannii reduce inflammation in septic rats, low doses of recombinant IL-17aAcinetobacter baumannii can aggravate inflammation in septic rats;3. high-doserIL-17a may possibly play a catalytic role in promoting PMN apoptosis by promotingCaspase3activation, s-Fas protein expression; PMN apoptosis the low-dose rIL-17amay play the role in inhibiting of PMN apoptosis by inhibiting the expression ofCaspase activation and s-Fas protein3.