Expressions And Significances Of CD44and CD133in Primary Lesion Of Gastric Cancer

Objective To investigate the expressions of CD133and CD44protein in primarylesions of gastric cancer and its clinical significance．Methods The expressions of CD44and CD133protein in the gastric cancer tissuesof100patients with gastric cancer were detected by immunohistocheimcalstainings． The relation between the expressions of CD44and CD133protein and theclinicopathologic characters were analyzed.Results Both CD44and CD133protein were expressed on the cell membranes.No correlation were found between CD44/CD133and the clinical clinicopathologicalparameters include gender and age （P＞0.05）, but the positive expression rate ofCD44/CD133with diameter＞5cm was significantly higher than that in the tumorwith diameter≤5cm (CD44P=0.150; CD133P=0.056), and correlated with thetissue differentiation (CD44P=0.008; CD133P=0.007), vascular invasion (CD44P=0.043; CD133P=0.023), lymphatic vessel invasion (CD44P=0.020; CD133P=0.044), lymph nodes metastasis (CD44P=0.002; CD133P=0.004), invasion depth oftumor (CD44P=0.006; CD133P=0.021), and pTNM stage (CD44P=0.034；CD133P=0.001). No correlation were found between the co-expression of CD44and CD133protein and the clinical clinicopathological parameters include gender, age, tissuedifferentiation, and vascular invasion （P＞0.05）, but the positive expression rate ofCD44/CD133with diameter＞5cm was significantly higher than that in the tumorwith diameter≤5cm (CD44+CD133+, P=0.010), and correlated with lymphaticvessel invasion (CD44+CD133+，P=0.003), lymph nodes metastasis (CD44+CD133+，P=0.045), invasion depth of tumor (CD44+CD133+, P=0.041), and pTNM stage(CD44+CD133+，P=0.049). The Spearman rank correlation analysis showed that therewas positive correlation between the expression of CD44and CD133protein (r=0.207,P=0.039). Univariate analysis showed that lymph nodes metastasis (P＜0.001), pTNM stages (P=0.013), CD44protein expression (P=0.005), CD133proteinexpression (P=0.002), and co-expression of CD44and CD133protein (P＜0.001) wassignificantly correlated with3-year survival rate of patients with gastric cancerrespectively．Logistic regression analysis revealed that lymph node metastasis (P=0.038)was independent risk factor for CD44or/and CD133protein expression. Multivariateanalysis with the Cox regression models showed that co-expression of CD44andCD133protein (P=0.003) and lymph node metastasis (P=0.006) were significantlyassociated with poor prognosis.Conclusions CD44and CD133protein may be considered as robust cancer stemcell markers in gastric cancer. The co-expression of CD44and CD133protein is theindependent prognosis factor for gastric cancer and strongly associated with poorprognosis when they are expressed more high.