| As an indispensable plasticizer, DBP is widely used in our daily life and industrial production. During the using process, DBP will be gradually released from the plastic products, and cause serious harm to human body through the direct or indirect contact and food chain bioaccumulation because DBP does not polymerize carbon chain. Diabetes is a common chronic diseases due to the of recession pancreatic islet function and insulin resistance. Diabetes has become the third non infectious disease for human health on the heels of cardiovascular diseases and cancer in the world wide range of distribution. The research has demonstrated that insulin resistance of the male is linked to the contact with PAEs. Based on this, the purpose of this study is to study the link of diabetes and the exposure of DBP, through the design of DBP exposure in Balb/C mice diabetes form.1. The type Ⅱ diabetes animal model under the effect of DBPBalb/C male mice model with type Ⅱ diabetes was succeedfully made by the method of the little dosage of STZ injection combined with high fat and sucrose feeding. The experiment was lasted for six weeks, and the Balb/C mice were randomly divided into eight groups, each group of eight after a week of the adaptive feeding. Among them, the single groups are the non-diabetic groups, the double groups are the diabetic groups and the1,2groups are the non DBP exposure groups, the3-8groups DBP exposure groups. During the experiment, the diabetic groups were feed in high-fat food, and injected with100mg/kg STZ solution in the third weeks. The non diabetic groups were feed in the ordinary food, and were injected with citric acid buffer solution in the third week. The DBP treated groups were given daily in the accordance with the requirements of the concentration of DBP, and the non DBP exposure group were given with the saline+Tween-80. After the experiment lasted for3weeks, the mice fasting blood glucose concentrations were measured through the tail clip, and after the normal feeding, the diabetic groups were intraperitoneally injected of100mg/kg STZ solution on the very next day. The non-diabetic groups were injected of the citric acid buffer solution. After one week, the fasting blood glucose values were measured continuously for three weeks, once a week. The mice diet, drinking volume and weight were weekly recorded. At the end of the experiment, the various indexes of the serum, liver, pancreas and other tissues were got to measure after the mice were sacrificed.2. The effect of DBP exposure on diabetesWe preliminarily studied on the effect for the formation of the type Ⅱ diabetes with the DBP exposure through the experiment of the DBP separate exposure groups and DBP exposure groups with combined type Ⅱ diabetic. In the present experiment, DBP exposure can cause the body tissue oxidative damage, and the damage and the DBP exposure concentrations have the dose effect relationship. DBP exposure can’t directly lead to type Ⅱ diabetes, but has certain stimulative effect for type Ⅱ diabetes formation. The way of action may be mediated by oxidative stress. |
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