Study On The Characterization And Identification For Resveratrol From Different Sources Of Raw Materials

Information of various kinds of chemical composition from Polygonum cuspidatum and Grape were collected in this paper. MS fracture patterns of five Anthraquinone compounds in Polygonum cuspidatum and Grape extracts were studied by UPLC-Q/TOF. This study will be useful to gain knowledge for Anthraquinone compounds in Polygonum cuspidatum and Grape, and can also contribute valuable information to the establishment of the compounds database of Polygonum cuspidatum and Grape. In addition, fingerprints for the resveratrol were estsblished basing on the data analysis of the chromatography of resveratrol samples for Polygonum cuspidatum source and Grape vine source. A comprehensive identification method concerning the distinguishing of Polygonum cuspidatum and grape vine source resveratrol was developed with the use of UPLC-Q/TOF technology. Furthermore, some characteristic fingerprint sections and marks were obtained to identify the Resveratrol sources.1Collection of the compounds database of Polygonum cuspidatum and GrapeInformation of previously reported chemical composition in Polygonum cuspidatum and Grape were collected. Two fractions in MS chromatography containing five Anthraquinone compounds in Polygonum cuspidatum and Grape were found based on the MS/MS technology. Results showed that the carbonyl of mother nucleus of Anthraquinone compounds is susceptive to cleavage, accompanying with a CO\CO2losing. The small molecular fragments on the Anthraquinone chemical structure are volunteered to be lose after electron collision, and the simple substituents are relatively preferred in the electron-induced bond breaking in MS by El source. The results would be helpful for the establishment of the compounds database of Polygonum cuspidatum and Grape.2Establishment of Fingerprints of the resveratrol extracts from Polygonum cuspidatum and Grape vine.Fingerprints for the resveratrol extracted from Polygonum cuspidatum and Grape vine were established with the use of UPLC-UV technology. Results showed that three characteristic fingerprint sections can be obtained by a comparative study on the difference in fingerprints between Polygonum cuspidatum and Grape vine.3UPLC-Q/TOF study on the Polygonum cuspidatum and Grape vine extractsA comprehensive identification method concerning the distinguishing of Polygonum cuspidatum and grape vine source resveratrol was established by UPLC-Q/TOF technology. Eleven compounds were identified according to MS information. Four identified compounds, including Physcion-1-O-β-D-glucoside、Emodin、Ampelopsin ε and Ampelopsin a, were found to be able to effectively distinguish the resveratrol extract of Polygonum cuspidatum source from that of Grape vine source. Measurements of MS data of Polygonum cuspidatum and Grape vine samples, and the verified original data by MPP software and statisical analysis found that11marks can be utilized to identify the RES of5%Polygonum cuspidatum source and5%Grape vine source, and7marks to identify the RES of98%Polygonum cuspidatum source and5%Grape vine source.4Adulteration validationThe ratio of RES/ε-viniferin and The TIC/UV spectrogram and PCA result from Polygonum cuspidatum and Grape extract and adulterate sample were studied and compared. Results showed that significant differences were existed between Polygonum cuspidatum and Grape extracts and the adulterate sample.