Studies On Effects Of Intestinal Regulation For Tea Extracts

Modern tea medical research shows that tea has many kinds of health benefits, like regulating intestinal flora and enhancing immunity. Although different teas may have the same health benefit, different materials, process technology, flavor and containing substances of teas lead to different efficacy and pathways of the same effect. This paper used six kinds of tea extracts lyophilized powder to feed mice, then inducing imbalance of intestinal flora and disorder of immune function in mice by feeding lincomycin HC1, attempting to explore the effects on regulating intestinal flora and immune function of different teas, and possible underlying mechanisms and regulatory pathways of this function. This study aims to provide a scientific basis for health care consuming of tea.The main results of the study are as following:1. Componential analysis of different tea extractsGreen tea, yellow tea and white tea have higher levels of tea polyphenols, amino acids; black tea has higher levels of amino acids and flavone, lower levels of tea catechins; oolong tea has higher levels of flavone and soluble sugar; dark tea has higher levels of soluble sugar, lower levels of tea catechins and caffeine.2. Study on prevention of different tea extracts on imbalance of intestinal flora in miceThis paper used the method of imbalance of intestinal flora animal model to evaluate the intestinal regulating effects of tea.The results showed that tea extracts can regulate the balance of Enterobacter, Enterococcus, Bifidobacterium and Lactobacillus in mice intestinal flora, increasing the splenic index, level of serum globulin and immunoglobulin IgG. The colon pathological section showed that there is no significant difference between high dosage groups of tea extracts and normal control group, but there is significant difference with model control group. Therefore, tea extracts has prevention effect to imbalance of intestinal flora in Mice induced by antibiotic.3. Study on mechanisms of the intestinal regulating effects of tea extractsThe mice small bowel of model group and high dose of different teas were choosed as samples. Selected three genes which related to intestinal immune.To investigate the changes of these genes’expression at the mRNA level, RT-qPCR analysis were performed. The result revealed that six kinds of tea extracts all have some impact on genes’expression at the mRNA level, down-regulated genes including TLR2, TLR4and NF-k B.