| In this paper, based on tetramethylpyrazine O/O Nano-aqueous micromusion, bychanging the internal oil phase, tetramethylpyrazine was made into O/O Nan-aqueousnanosuspension loading with tetramethylpyrazine. And also study the stability of it.The differences of tissue distribution after oral administration of tetramethylpyrazineO/O nanosuspension and water suspension were investigated. Compared to the watersuspension, the bioavalibility was improved.By studying the MTT of Caco-2, the proper of tetramethylpyrazine concentration wasselected. From the study of different effecting factor on the uptake of Caco-2, theoptimal temperature, time and PH value were selected. The uptake oftetramethylpyrazine was basic line increased as the concentration increased, and therehas no saturation phenomena and indicated the passive transport is the main absorptionmechanism.The brain injury model of AD rats was made by injecting Aβ protein in hippocampusof rats. The brain tissues were completely removed after perfusion in vivo withparaformaldhyde, then wax sealing, slicing and so on. We can made judgment on thearea of CA1of hippocampus directly by Nissl Wright staining. Compared with modelgroup, the ratio of TNF-α, NOS1and NF-Κb which were expressed on CA1area ofhippocampus were made. And from the ratios, the effect of protecting against the braininjury were visually displayed.In this paper, an HPLC method was developed and validated to measure thetetramethylpyrazine concentration in plasma, heart, liver, spleen, lung, kidney, brainand Caco-2. The differences of the distribution in tissues and the differences of uptakein Caco-2cells and also the protected function of brain injury of AD rats were discussed in this paper. |
