Studies On Active Ingredients And Fingerprint Of Ganoderma

The present situation of Ganoderma cultivation is multi–species in our country, andcultivation methods and conditions are different, at the same time, geographicalenvironment, climate, temperature and other factors vary greatly. It is not clear if thesefactors will affect the nutrients and active substances of the fruiting body, and bringabout changes to the quality of Ganoderma lucidum. Therefore, it is necessary toestablish the detection method of the active ingredient in order to examine the influencedegree of the active ingredient.It is first time to establish a HPLC method for detecting GLIS, which is an activepolysaccharides with high molecular weight isolated from the fruiting bodies ofGanoderma lucidum. The polysaccharide GLIS was separated with the other ingredientsusing HPLC analysis, methodological study test showed good repeatability, highprecision, good reproducibility, which is suitable for the quantitative analysis of theactive polysaccharide of Ganoderma. Ganoderma samples of different sources wereanalyzed, the results showed that only a portion of Ganoderma lucidum contains theactive polysaccharide GLIS. But it does not detected in the Ganoderma sinense,Ganoderma applanatum, Ganoderma tenus and Ganoderma tsugae.The chromatographic conditions of HPLC fingerprint are optimized by comparingdifferent column and mobile phase. The optimized HPLC fingerprint contained acidicGanoderma triterpenoid peaks and neutral Ganoderma triterpenoid peaks. Themethodological study results show it had good repeatability, precision andreproducibility with the compliance analysis requirements for fingerprint analysis.Different sources of Ganoderma sample were analyzed using the fingerprint method theresults showed the similarity of Ganoderma different species fingerprint is very low,and its main triterpenoid ingredient content was significantly different. Some of the different varieties of samples had low similarity, and there also some samples had highsimilarity. The similarity between different cultivation methods, cultivation locations,cultivation of age or harvest time harvest Ganoderma sample are very high, indicatingthat these factors did not effect the content of Ganoderma Triterpenes.HPLC method was established to detect the content of uracil nucleosides, uridine,adenine, and guanosine of Ganoderma. This method is simple, rapid, and good stabilityand repeatability. The contents of four nucleosides from different species of Ganodermahad significant difference through HPLC analysis, there are also significant differencesin varies species of Ganoderma. HPLC fingerprint analysis method of Ganodermanucleotidoids were established, and was used to detect different sources of Ganoderma,it is found that the content of nucleosides significantly reduced with the extension ofspore powder collecting time, particularly the stain sample of109, which is differentwith the results of triterpenoid. The similarity of Ganoderma species such asGanoderma sinense, Ganoderma applanatum, Ganoderma tsugae, Ganoderma tenusfingerprint is lower, these factor of different cultivars, cultivation time, place ofcultivation had effect on the content of Ganoderma nucleosides.The quantitative determination HPLC method of active polysaccharide were firstestablished, HPLC triterpenes fingerprint condition of were optimization, and HPLCnucleosides fingerprints was first established to detect different sources of Ganoderma.These research establish a basis for the Ganoderma quality control.