Protective Effects Of Inhibiting NgR On Diabetic Retinal Ganglion Cells

ObjectiveTo explore the effects of the NgR on the apotosis of Diabetic retinalganglion cellMethodsDiabetic rats were induced by a single intraperitoneal injection of(streptozotocin STZ,50mg/kg), and blood glucose concentration from caudalvein more than16.7mmol/l were considered diabetic Experiments divide into4groups：siNgR group(inject10μl NgR siRNA virus into the vitreous cacity ofdiabetic rat’s echo eyes),NU group (inject10μl virus negetie control fluid intothe vitreous cacity of diabetic rat’s echo eyes),DM group (inject10μl virusdiluent into the vitreous cacity of diabetic rat’s echo eyes),CON group (inject10μl virus diluent into the vitreous cacity of diabetic’s echo eyes). feed after12weeks. Apply immunofluorescence and HE to detect the expression ofNgR. Apply Western Blot to detect the quantity of NgR, and Apply TUNEL todetect the apoptosis of every group.ResultsThe result of immunofluorescence to show that NgR height expression inretinal ganglion cells layer. The result of Western Blot show that, comparewith control, the group of NU、the group of DM over-active（P<0.01）.Theresult of HE show that the RGC of CON have a regulation shape，andarrangement is consistent.the cell of DM and NU have a no regulation shape， arrangement is disorder and the number is decrease. While the group ofNgR compare with NU show that the retinal cells have a regulation shape，arrangement is consistent and the number is increases. the result of TUNELshow the group of DM and NU apper the obvious apoptosis cell, and thegroup of CON and NgR have a no obvious apoptosis cell.Conclusions1. The protein of NgR have a over-active in diabetic retinal ganglion cellslayer.2. siRNA can effectively inhibit the expression of NgR in diabetic mellitusrats RGC.3. The DM rat retina NgR upregulated accompany with RGC apotosis,and the inhibition of NgR expression inhibits DM rat RGC apoptosis, explainNgR is one of the important mechanisms of the DM rats RGC apotosis.