The Study Of Allogeneic Costal Chondrocyte To Repair The Full-thickness Cartilage Defect On The Knee Of The Rabbit Model

objective:discussing the effects of Allogeneic costal chondrocyte/Sodium hyaluronate composites to repair the full-thickness cartilage defect in the knee of the rabbit model.Methods:costicartilage obtained from rabbits was cut into pieces by mechanical method. Isolate and culture chondrocyte by cell culture techniques and tissue culture techniques. Costal chondrocytes was identified by inverted phase contrast microscop and immunocytochemical staining, Choose the right generation for cartilage tissue engineering research.Made full-thickness cartilage defect models on the knee of rabbit(n=36), allocate to blank, control and experimental groups randomly (n=12). The blank group was subjected to no disposal, the Sodium hyaluronate was injected into the defects of the control group, and costal chondrocytes/Sodium hyaluronate composites was injected into the defects of the experimental group. Every4rabbits were killed randomly by the end of the first, second and third month respectively, Obtain specimens from distal femur. Observe the specimen tissue with naked eyes and histological staining and get score in O’Driseoll, keeley and salter method.Results:The original cells and first generation costal chondrocyte showed typical morphological characters. The morphology of chondrocyte from the second to the fourth generation got longer gradually. While, from the fifth generation most of them became spindle and transformed into fibroblasts. The staining of alcian blue, safranin O-solid green and Type II collagen of costal chondrocytes of the second generation were all strong positive.The cartilage defects of blank group and control group were repaired mainly by fiber tissue, while the experimental group was repaired by cartilage tissue, and the staining of H-E, alcian blue, safranin O-solid green and Type Ⅱ collagen were all strong positive.The score of O’Driseoll, keeley and salter:First month:the blank group and control group were not be repaired, no difference between each other (P>0.05), while the experimental group has obvious cell repairment (P<0.05). Second month:experimental group ranked first, followed by control group and blank group, obvious difference between each other (P<0.05). Third month:experimental group ranked first, followed by control group and blank group, obvious difference between each other (P<0.05). Blank group: the score decreased in time-dependent manner, but no difference between each other (P>0.05). Control group:the score increased in time-dependent manner, obvious difference between each other (P<0.05). Experimental group:the score increased in time-dependent manner, obvious difference between each other (P<0.05).Conclusion:The second generation Costal chondrocytes is the right generation for cartilage tissue engineering research. Allogeneic costal chondrocyte/Sodium hyaluronate composites can repair the full-thickness cartilage defect on the knee of the rabbit model, and the result was satisfactory. Figure72, table5, references57.