Study On The Relationship Between The Change Of Exp Ression Of Wntβ-catenin, Wnt10b In Callus And Type2Diabetes Fracture Healing Disorder

Objective:To establish fracture traction model of type2diabet es rats and to discuss the relationship between expression changing of Wntβ-catenin, Wnt10b in the callus and diabetes fracture healing disorder.Methods:To divide30healthy SD rats with six weeks age into2groups with the same numbers by randomly,one group is name d experiment, the other is control group.The rats in experient group will be feed with high amount of sugar and fat,while the rats in c ontrol group will be raised with common feed. After eight weeks,ea ch rat will be evoked by abdominal cavity Injection with STZ(30m g/kg) in experiment group, while the rats will be injected by citrate with the same quantity and same way in control group. Two more weeks later, to select SD rats whose blood glucose is higher than16.7mmol/L in empty stomach state, then continue to carry on the following experiment and establish left side tibia fracture traction m odel in each group at the same time. Then to lengthen the left tib ia from the second day to the14th day after the operation with0.3mm/day and twice time per day. It stops to lengthen at the15th day and kill all the rats after collecting the serum sample, to colle ct left tibia under aseptic conditions and examine X-ray radiography immediatedly.To test TG, TC, FINs, FBG in serum samples at8th week,10th week and before killing. Observing callus generation in HE staining and to histologically quantitative analize forming area of new bone in callus and the number of fat cells per unit in the bone marrow cavity. To do Western blot and Real-time fluorescence Quantitative detection System for left tibia callus sample. Results.-After raising eight weeks,serum FINS, TG and TC in experirental g roup increased(P<0.01) comparising to the control group, FBG with no obvious difference(P>0.05);After raising10weeks and the over time of experient, TG, TC and FBG in experiental group increased (P<0.01) comparising to the control group, while FINs with no ob vious difference (P>0.05); X-ray radiography showed that the form ing callus in traction space of experiental groud obviously decreased comparising to the control group.The callus tissue HE staining sho wed that the MCF of experiental group obviously decreased compar ising to the control group with disordered arrangement, shorter and smaller.And the PMF decreased. While histological quantitative anal ysis to callus showed that forming size of new bone in callus in e xperiental group decreased comparising to the control group while t he number of fat cells per unit in the bone marrow cavity increase d (P<0.01). W-B and QPCR detection showed that Wntβ-catenin ex pression in callus decreased in experiental group(P<0.05) comparisin g to the control group and the same with Wnt10b(P<0.05).Conclusion:Type2diabetes rats healing disorder after fracture, the decrease of Wntβ-catenin, Wnt10b expression in callus and cla ssic Wnt signaling pathway has been restricted which may be one of the reasons causing type2diabetes fracture healing disorder.