Effect Of Velocity Of Anticoagulant And A New Type Suction Tip On The Quality Of Erythrocyte In Autologous Blood

ABSTRACT Objective:To observe the impact of anticoagulant at different velocities on the quality of erythrocyte in autologous blood using traditional suction tip or a new type suction tip. To explore the most appropriate velocity of anticoagulant for minimizing hemolysis during blood cell salvage.To compare the influence of two kinds of suction tip on the quality of erythrocyte in autologous blood.Methods:1. Collect surgical drainage blood within6hours after orthopedics operations. Drainage blood was randomly divided into3groups (each group contains15cases):A group (the velocity of anticoagulant is lml/min), B group (the velocity of anticoagulant is2ml/min), C group (the velocity of anticoagulant is4ml/min). Each group sets three different velocities of blood flow:A1,B1,C1(V=2ml/min), A2,B2,C2(V=4ml/min), A3,B3,C3(V=8ml/min). Build a model using traditional suction tip and double-lumen tube with negative pressure. Observe the morphology, osmotic fragility and hemolysis of RBC after suctioning.2. Further collect surgical drainage blood within6hours after orthopedics operations. Drainage blood was randomly divided into3groups (each group contains15cases):A’group (the velocity of anticoagulant is lml/min), B’group(the velocity of anticoagulant is2ml/min),C’group(the velocity of anticoagulant is4ml/min). Each group set three different velocities of blood flow:A1’, B1’, C1’(V=2ml/min), A2’,B2’, C2’(V=4ml/min), A3’, B3’, C3’(V=8ml/min). Build a model using a new type suction tip and ordinary tube with negative pressure. Observe the hemolysis of RBC after suctioning.Results:1.Using tradition suction tip:1)morphology score was signigicantly increased after suctioning compared to before suctioning in each group(p<0.05);there was significant difference among A1,A2,A3(A1<A2<A3,p<0.05); there was significant difference among B1,B2,B3(B1<B2<B3, p<0.05); there was significant difference among C1,C2,C3(C1<C2<C3, p<0.05); there was significant difference among A1,B1,C1and the highest score in A1(p<0.05); there was significant difference among A2,B2,C2and the lowest score in C2(p<0.05); there was significant difference among A3,B3,C3and the lowest score in C3(p<0.05).2)Osmotic fragility was significantly increased after suctioning compared to before suctioning in each group(p<0.01); there was significant difference among A1,A2,A3and the highest osmotic fragility in A1(p<0.05); there was significant difference among B1,B2,B3and the highest osmotic fragility in B1(p<0.05); there was no significant difference among C1,C2,C3in osmotic fragility (p>0.05). There was significant difference among A2,B2,C2and the lowest osmotic fragility in A2(p<0.05); there was significant difference among A3,B3,C3and the lowest osmotic fragility in A3(p<0.05); there was no significant difference among A1,B1>C1in osmotic fragility (p>0.05).3) There was no significant difference among groups at the same velocity of anticoagulant in hemolysis after suctioning(p>0.05); there was significant difference among A1,B1,C1and the lowest hemolysis in B1(p<0.05);there was significant difference among A2,B2,C2and the lowest hemolysis in B2(p<0.05); there was significant difference among A3,B3,C3and the lowest hemolysis in B3(p<0.05).2. Using new type suction tip:there was no significant difference among groups at the same velocity of anticoagulant in hemolysis after suctioning(p>0.05); there was significant difference among A1’ B1’C1’ and the lowest hemolysis in A1’(p<0.05); there was significant difference among A2’ B2’C2’ and the lowest hemolysis in A2’(p<0.05); there was significant difference among A3’B3’C3’ and the lowest hemolysis in A3’(p<0.05);3. There was significant difference between A group and A’group at the same velocity of anticoagulant and the same velocity of blood flow in hemolysis(A1’<A1, A2’<A2, A3’<A3,p<0.01); there was significant difference between B group and B’group at the same velocity of anticoagulant and the same velocity of blood flow in hemolysis(B1<B1’, B3<B3’,p<0.05).Conclusions:1.The most appropriate velocity of anticoagulant is2ml/min using traditional suction tip for minimizing hemolysis.2. The most appropriate velocity of anticoagulant is1ml/min using the new type suction tip for minimizing hemolysis.3. The velocity of blood flow does not influence hemolysis but to some extend influences the morphology and osmotic fragility of RBC in autologous blood.