The Experiment Research On Mechanism Of Radiation-induced Lung Injury And Prevention And Therapy Of Matrine

Objective: In this paper，use Wistar rats as the research object, use matrine that hasa certain role in the Radiation-induced lung injury treatment and is widely used in thetreatment of cancer, observe before X-ray irradiation and after, and after matrineinterfering, lung tissue TGF-β1, CTGF and collagen expression prognosis, to explorethe possible mechanism of Radiation-induced lung injury and the preventive andtherapeutic effect of matrine.In order to provide valuable experimental data for clinicalprevention and treatment of radiation-induced lung injury, and provide reference forclinical application of Chinese medicine in the prevention and treatment ofradiation-induced lung injury.Methods:40female Wistar rats, were randomly divided into No-treatment Controlgroup, Radiotherapy Control group, Matrine Control group, Preventive treatment group,Drenching treatment group, Combining prevention with control group. In addition to thefirst two groups, the other four groups by X-ray dose of15Gy irradiation for3minutes,and construct the model of Radiation-induced lung injury; At the same time, make amatrine intervention. The5μm slice is Completely lost-wax in xylene, dehydrated ingraded ethanol. HE and Masson staining in order to semi-quantitative determine thedegree of alveolitis and pulmonary fibrosis; oberserve expression of collagen.Immunohistochemical detection of cytokines in lung tissue of TGF-β1, CTGF. Degradeaccording to the degree of Radio-pulmonary lesion，the expressional intensity ofcytokine and collagen，finishing a level data for statistical analysis, statistical resultsare in P<0.05with significant difference. Results:(1)After X-ray irradiation, the rats from all groups showed differentdegrees of Radiation-induced lung injury. Among them,7d-14d mainly showedalveolitis;14d-21d Alveolitis is reduced gradually, but pulmonary fibrosis aggravatedgradually;after28d, mainly to pulmonary fibrosis;60d lung volume changes smaller,has white spots on the surface, and appeared different degrees of pleural adhesions.(2)The pulmonary fibrosis expression of TGF-β1, CTGF in lung tissue was strongestafter14d radiotherapy (+++), with stable, TGF-β1, CTGF expression decreased (++),and tends to be stable.(3)By HE, Masson staining semi-quantitative determination ofmatrine intervention group rats look better in alveolar inflammation, pulmonary fibrosisthan the control group (P<0.05).(4)Compared with radiotherapy group, matrine caninhibit the expression of lung tissue in TGF-β1, CTGF.(P<0.05).Conclusion:1.After14d X-ray irradiation, alveolitis gradually transition topulmonary fibrosis, after radiotherapy of28d, pulmonary fibrosis tends to be stable, sothe ideal model of pulmonary fibrosis was established at this time.2. TGF-β1andCTGF are related closely to Radiation-induced lung injury, especially early alveolitisand pulmonary fibrosis.3.After the matrine intervention, for rats in each group，Radiation-induced lung injury is reduced, and combining prevention with control groupwas better than that of the treatment group,and the treatment group was better than thepreventive treatment group, its mechanism may be achieved through improving theoxidative stress state in Radiation-induced lung injury rats body.4. After radiotherapy of42d, even though the pulmonary fibrosis tends to be stable, use matrine to intervene canreduce the degree of pulmonary fibrosis, and its mechanism may be to reduce collagenproduction by inhibiting expression of TGF-β1and CTGF.