Small Interfering RNA Inhibition Of CTGF Attenuates The Effect Of Transforming Growth Factor Betal On CTGF, VEGF And Extracellular Matrix In Cultured Human Peritoneal Mesothelial Cells.

Peritoneal fibrosis is one of the most serious complications of long -term continuous ambulatory peritoneal dialysis treatment.Peritoneal fibrosis can cause decrease of ultrafiltration efficacy , eventually can lead interrupt of peritoneal dialysis.The mechanisms of peritoneal fibrosis is not well defined,It is thought that the bioincompatible solution and its related products,acute episodes of peritonitis and cytokines are the major causes .In recent years ,a large number of studies have showed the profibrotic growth factor,transforming growth factor-β1 (TGF-β1) plays a key role in mechanism of peritoneal fibrosis.In addition to its profibrotic activity, TGF-β1 plays a pivotal role as a modulator of immune function and tumor growth, raising concern about the long term consequences of its suppression.Connective tissue growth factor (CTGF) is a downstream factor of TGF-β1.As its stimulation of cell migration and matrix production are mediated through TGF-β1,providing an attractive target molecule for the modulation of matrix overproduction in peritoneal fibrosis.The RNAi technique has become a powerful and widely used tool for the analysis of gene function and gene therapy. Broadly speaking, RNAi technique can lead to gene knockout or gene silencing. So,considering the role of CTGF in the mechanism of peritoneal...