An Experimental Study Of Xie’s Liver Cancer Prescirption NO.2on Mice With H22Liver Cancer

Objective: The onset of primary liver cancer (PLC, be hereafter referred to as livercancer) is invisible. As usually there is no symptom at an early stage,they will beexamined by advanced liver cancer after they go to the doctor. In normal situation, thesurvival of advanced liver cancer is from3to6months. As radiation and chemotherapyare not very effective to liver cancer, until now the main treatment is surgery. However,when diagnosed, most patients have lost the chance of operation.The traditional Chinesemedicine (TCM) is very potential for the treatment of liver cancer and it is meaningfulto take the advantage of TCM.The purpose of this experiment is to study and prove thebetter anti-tumor and immunoregulation effect of Xie’s liver cancer prescriptionNO.2。Methods: reanimate liver cancer cell H22, make it off centre, collect the cell into1MLof saline water and mix well, then separate in2parts and inject in the abdominal cavityof two BALB/C mouse. After one week-raise get the ascites of the mice to prepare forthe model. Dilute physiological saline in cell suspension which consistency is1×107/mL, and inoculate each mice0.2mL under subcutaneous tissue of right axillary. Thenprepare another40BABL/C mouse（18-22g) which include20males and20females,and inoculate0.2mL under their subcutaneous tissue of right axillary(the number oftumor cell is about2×106) to reproduce the H22liver cancer mice model. The nextday of experiment(24hours after model set),divide the inoculated mouse into4groupsrandomly, including model set group, Xie’s liver cancer on the2ndgroup, HerbaScutellariae Barbatae group and IL-2group and each group has10mouse. Model setgroup: drench0.4ml of physiological saline; Xie’s liver cancer prescription NO.2group:using1ml of liquid medicine as1.5g of crude drug, drench the mouse0.4ml each day; IL-2group: inject0.2ml into the abdominal cavity of each mice using140000u/kg ofmedicine (equal to20times of daily dosage for adult in clinic); Scutellaria barbatagroup: using1ml of liquid medicine as1.5g of crude drug, drench the mouse0.4ml eachday. Do the experiment for14days. On the15th day, kill the mouse, get the tumor outto Weigh the weight of tumor,calculate the tumor inhibition rate, thymus index, spleenindex and other related indexes and detect proteins of Bcl-2and Bax throughimmunohistochemistry.Results:1.Effect of Xie’s liver cancer prescription NO.2on weight change. Xie’s livercancer prescription NO.2group，Herba Scutellariae barbatae group and IL-2group werehigher than that of model group increased body weigh（tp<0.05）. And Xie’s liver cancerprescription NO.1group and IL-2group weight increased more obviously.2. The effect of Xie’s liver cancer prescription NO.2to tumors’ growth inhibitionratio. Xie’s liver cancer prescription NO.2group, Scutellaria barbata group andIL-2group on tumor-bearing mice tumor have different degrees of inhibition. The dataof Xie’s liver cancer prescription NO.2group is similar as that of Scutellaria barbatagroup, and both of the two group get the higher rate than IL-2group’s.3.Effect of Xie’s liver cancer prescription NO.2on immune organ index.Theresults show：No significant differences in Xie’s liver cancer prescription NO.2groupand IL-2group, the thymus index and spleen index (p>0.05)， they were bothsignificantly higher than that of Herba Scutellariae barbatae group and model group(p<0.05).4.The influence of Xie’s liver cancer prescription NO.2to Tumor-bearing mice cellapoptosis. The Bcl-2content of Xie’s liver cancer prescription NO.2group is similar asthat of Scutellaria barbata group, and lower than that of model set group and IL-2group. The content of Bcl-2of model set group is the highest while the result of Baxcontent is the opposite. Both data of Xie’s liver cancer prescription NO.2group andScutellaria barbata group are higher than the other two groups’ and the content ofmodel set group is the lowest.Conclusion: Obtained through the experiment, Xie’s liver cancer prescription NO.2canimprove the survival situation of Tumor-bearing mice，increase the body weight of miceand effectively inhibit the tumor. Also, it can lower the Bcl-2’s gene expression ofTumor-bearing mice with liver cancer H22and upper its gene expression of Bax.It cansignificantly improve the immune function, alleviate the damage to the body of malignant tumor, make human body and the cancer long-term coexistence, mutualnon-aggression. This experiment provides a solid theoretical basis for the clinical"survival" concept.