Biochemical Analysis Of Intervention Of Resveratrol On The Oxidative Damage Induced By Environmental Factors

When subjected to various noxious stimuli, the body produced too much highlyreactive molecules （rfee radicals） such as reactive oxygen species （ROS） and reactivenitrogen species （RNS），which could make the body’s internal macromolecular nucleicacids, lipids, proteins suffer oxidative damage and then induce multiple diseases, forinstance, obesity, diabetes, skin diseases, aging and so on. While oxidative damage canbe generated through a variety of metabolic pathways, for example, chemical toxins,drug metabolism, cell respiration, radiation, light, etc.In recent years, effects of natural products on protecting cells rfom oxidativedamage have aroused attention from more and more researchers. Resveratrol is just oneof them. It is a natural polyphenol extracted rfom the plants. Studies have shown that ithas a series of good effects on anti-cancer, anti-inflammatory, anti-viral andanti-oxidation in human body. Therefore，resveratrol has become a hot area of researchand is expected to be developed into a natural medicine used in clinical therapy.In this study, we place mode cell HeLa for the object, adriamycin, ethanol andUVC were selected to induce oxidative damage separately for drug metabolism,chemical toxin and radiation metabolic pathways, study the intervention of resveratroltowards oxidative damage induced by environmental factors, and take advantages oftools on cellular level, biochemical analysis and molecular biology level to elaboratebest intervention of resveratrol on cellular oxidative damage by environmental factorsto provide theoretical support for the application of resveratrol. The main results are asfollows:1.Research on repair function of resveratrol on adriamycin-indueed oxidativedamage. Use MTT assay to select adriamy c in-indue ed injury model （2μg/mL,12h） andoptimal intervention of resveratrol （50μM，repair for24h，the survival rate increased25.23%）. Results in the cellular level, biochemical and molecular biology analysishave proved resveratrol repair adiramycin-induced oxidative damage by increasingreducing substance or decreasing oxidizing substance, which have providedtheroretical basis and implementation suggestions for resveratrol to be used to reduceside effects of drugs.2.Research on repair function of resveratrol on ethanol-induced oxidativedamage. Use MTT assay to select ethanol-induced injury model （4.8%，12h） andoptimal intervention of resveratrol （20μM，repair for24h，the survival rate increased 9.6%）. Results in the cellular level, biochemical and molecular biology analysis haveproved resveratrol repair ethanol-induced oxidative damage by increasing reducingsubstance or decreasing oxidizing substance, which have provided theroretical basisand implementation suggestions for resveratrol to be used to reduce side effects ofethanol.3. Research on protection function of resveratrol on UV-induced oxidativedamage. Use MTT assay to select UV-induced injury model (400J·m^-2) and optimalintervention of resveratrol （50μM，protect for30min，the survival rate increased59.5%）. Results in the cellular level, biochemical and molecular biology analysis haveproved resveratrol protect cells from UV-induced oxidative damage by increasingreducing substance or decreasing oxidizing substance, which have providedtheroretical basis and implementation suggestions for resveratrol to be developed intoa related drug or an additive in skin care products.