| Aging is a progress that the physiological functions degenerate gradually, and in the agingprogress, there are some age-related diseases, such as osteoporosis, cardiovascular disease,arthritis, atherosclerosis, Alzheimer’s disease and malignant tumor. In nowadays, with theimprovement of living standard and medical services, more and more people begin to focus onhow to retard aging progress and prolong lifespan. Therefore, it is significant to prevent theage-relate diseases and perform the anti-aging related studies.Resveratrol is a natural polyphenol mainly derived from grape. There were manyexperiments demonstrated that resveratrol could extend the lifespan of diverse biological modelssuch as yeast, elegans and flies. The annual fish Nothobranchius with the biologicalcharacteristics of short lifespan and accelerated growth is easy to raise and reproduce in a largescale. Many studies revealed that Nothobranchius exhibited the expressions of aging relatedbiomarkers at the behaviour and histological level. Therefore, Nothobranchius is becoming anew vertebrate model, which can be used to perform the aging and anti-aging related studies.The previous results of our laboratory showed that resveratrol extended the mean lifespanand maximum lifespan of Nothobranchius guenther by19%and28%; and the research ofGenade also showed that resveratrol could extend lifespan of N. guentheri. However, themechanism remains unclear. In order to investigate the regulation mechanism of resveratrol onlifespan, we studied the markers of oxidative stress in N. guentheri.In this study, the fishes of N. guentheri were divided into two groups, resveratrol-treatmentgroup and control group at16weeks of age. The fishes of resveratrol-treatment group were fedwith the food included resveratrol and the fishes of control group were fed with the food withoutresveratrol. We observed the effect of resveratrol on aging progress and lifespan, measured thechanges of the oxidative stress markers to study the mechanism of resveratrol on lifespan in N.guentheri. The results showed: 1. Resveratrol extended the mean lifespan by17.34%and maximum lifespan by17.66%.For the fishes of different genders, resveratrol both prolonged the mean lifespan and maximumlifespan in males and females. Obviously, contrasting to the fishes of female, resveratrol had asignificant effect on male fishes, this maybe relate to the drug dose and gender difference. Wechose the male fishes of N. guentheri to perform the follow study.2. To investigate the effect of resveratrol on the oxidative status of N. guentheri, wemeasured the markers of oxidative stress including reactive oxygen species (ROS) level andantioxidant enzymes activities at the biochemical level. The results here revealed that the ROSlevel increased and antioxidant enzymes activities decreased significantly with age in the N.guentheri at6,9and12months (young, middle-age and old); the activities of the threeantioxidant enzymes including catalase (CAT), superoxide dismutase (SOD) and glutathioneperoxidase (GPx) increased and the level of ROS decreased significantly byresveratrol-treatment.3. The change of ROS level could affect the oxidative damage of macromolecules.Contrasting to the alter of ROS level at6,9and12months, we detected the oxidative damageand the accumulation of lipofuscin of N. guentheri at biochemical and histological level. Wefound that the oxidative damage of protein, lipid and lipofuscin increased significantly with agein the N. guentheri; resveratrol decreased the upward trend of protein oxidation, lipidperoxidation and lipofuscin accumulation induced by aging. That was to say, resveratroldecreased the oxidative stress of N. guentheri.In conclusion, resveratrol extended the lifespan of N. guentheri and attenuated the changesof oxidative stress markers. Together with these results found in N. guentheri, we speculated thatthe decline of oxidative stress played an important role in the lifespan extension progress ofresveratrol. |
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