The Study On Heat Shock Protein60and70Induction By Teprenone And Its Protective Effect On Acute Gastric Mucosal Lesion In Severely Burned Patients

Objective：by observing the changes of burn induced-gastric mucosal lesionthrough gastroscope and detecting the expressions of Heat shock protein （HSP）60and70in gastric mucosa of severely burned patients using teprenone, to investigatethe protective effects of teprenone and HSP60and HSP70on acute gastric mucosallesion in severely burned patients.Method:. Burned patients were divided into teprenone treatment group(15persons, T group), conventional treatment group(15persons,C group)and normalcontrol group(10persons, N group).Gastric mucosal lesion was observed throughgastroscope in teprenone treatment group and conventional treatment group withinadmitted to hospital one week and four weeks respectively. Immunohistochemistrywas used to detect HSP60and HSP70expressions after endoscopy collection ofgastric mucosa. Realtime-PCR method was used for detection of HSP72mRNAexpression of gastric mucosa in each group.Results：1. The endoscopy and HE staining showed that the degree of gastricmucosal injury significantly reduced than that of the conventional treatment group.2.Immunohistochemistry results showed that: the level of HSP60expression of Tgroup (0.1427±0.0149) was significantly higher than that of C group (0.1122±0.0118) and N group (0.0406±0.0037) in first week, the difference was statisticallysignificant (P<0.05). The level of HSP60expression (0.0468±0.0051) in fourthweek is slightly higher than that of C group (0.0433±0.0045) and N group(0.0406±0.0037), no significant difference (P>0.05)was found in fourth week after burn. Thelevel of HSP70expression of T group (0.1587±0.0162) in first week after burn wassignificantly higher than that of C group(0.1281±0.0131) and normal control group(0.0402±0.0038), the difference was statistically significant (P<0.05). HSP70expression level of T group (0.047±0.0048) in fourth week after burn is slightlyhigher than that of C group (0.0439±0.0044) and N group(0.0402±0.0038),Therewas no significant difference among three groups(P>0.05).2. Real-time PCR results showed that: HSP72mRNA expression level in T group (1.002±0.061) was significantly higher than that in C group (0.797±0.049)in the first week after burn and that in N group （0.312±0.033）, the difference wasstatistically significant (P <0.05). HSP72mRNA expression level in T group（0.355±0.028）slightly higher than that in C group（0.338±0.024）after scaling fourweeks and than that in N group （0.312±0.033）, the difference was not statisticallysignificant (P>0.05).Conclusion: Gastric mucosal lesion in T group was significantly lower than thatin C group (P<0.05), showing that teprenone was of protective role to acute gastricmucosal lesion in those severely burned patients. The expressions of HS60and70inT group was higher than that in C group (P<0.05), which illustrate HSPs inducersteprenone might involve in gastric mucosal protection in scalded patients throughenhancing the expressions of inducible HSPs. This study might provide clinicalevidence for using teprenone to cure acute gastric mucosal lesion caused by burn.