The Expression Of Dysadherin And E-cadherin In Cervical Squamous Cell Carcinoma And Its Significance

Objective:1. To observe the expression changes of dysadherin and E-cadherin protein incervical squamous cell carcinoma and their clinical significance and to analysis therelationship between the expression and clinicopathologic factors.2. To explore the expression of mRNA of dysadherin and E-cadherin in cervicalsquamous cell carcinoma.3. To analyze the relationship between the expression of protein and mRNA ofdysadherin and E-cadherin in cervical squamous cell carcinoma and to preliminarilystudy the role of dysadherin E-cadherin in the development of cervical cancer.Methods:Immunohistochemical method was used to detect the protein expression ofdysadherin and E-cadherin of62cases of cervical squamous cell carcinoma and30cases of normal cervical tissues. The RT-PCR method was used to detect the mRNAof dysadherin and E-cadherin of32cases of cervical squamous cell carcinoma and21cases of normal cervical tissues.Results:1. The dysadherin protein expression rate(64.5%，40/62) was significantlyhigher in cervical cancer than in normal cervical tissues(43.3%，13/30). Theexpression was related with pathological grade, clinical stage, myometrial invasionand the presence of lymph node metastasis of cervical cancer (P<0.05). Theexpression rate of E-cadherin was down in cervical cancer group (40.3%，25/62)than in normal group(90.0%，27/30)(P＜0.05), the expression was related withpathological grade, clinical stage, and the presence of lymph node metastasis ofcervical cancer (P <0.05). There was negative correlation between the expression ofprotein of dysadherin and E-cadherin(r=－0.352, P=0.005,<0.01).2. The mRNA relative expression values of dysadherin in Cervical cancer group(1.1668±0.1898) was higher than which in the normal group (0.8103±0.0379), andmRNA expression of E-cadherin in cervical cancer tissue (0.7840±0.0541) was significantly lower than normal tissue (0.9660±0.0786). There was negativecorrelation between the expression of mRNA of dysadherin and E-cadherin(r=－0.689, P=0.000,<0.01).Conclusion:1. The protein expression of dysadherin is up-regulated, the protein expression ofE-cadherin is down-regulated in cervical cancer tissues, both of the expressions areclosely related to tumor invasion and metastasis.2. The mRNA level of dysadherin and E-cadherin are basically corresponding toits protein expression, indicating that post-transcriptional regulation of dysadherinexpression plays only a minor role in cervical carcinoma.3. Both of mRNA and protein expression of dysadherin and E-cadherin incervical cancer are negatively correlated, The combination detection of both can beused as a potential marker for cervical malignant degree.