| Objective:Glioma is the highest malignant type of intracalvarium tumor which has invasivegrowth characteristics, surgery is difficult to completely removed, and easy toelapse.Patients with this tumor are not treated after diagnosis patients,with a meansurvival of only a few months. Presently，although integrated treatment with surgery，radiotherapy, chemotherapy, immune, and also comprehensive treatment are taken todeal with glioma，the overall therapeutic efficacy of glioma is still unsatisfactory.Invasion is a malignant biological characteristics of glioma,and is also the importantreason for poor prognosis of glioma.It is noteworthy that the invasion of glioma is controlled by multiple importantMachinerie. The Invasion of glioma has significant correlation with the expression ofp65which is a member of the nuclear factor kappa B (NF-κB) family. the activatedNF-κB can promote the transcription of CB and MMP-9gene,so that the processwas promoted by hydrolysis of extracellular matrix ability was enhanced. So theNF-κB can offer a inhibiting targets of anti-invation in human glioma.Triptolide is a chemical composition separated from the Chinese planttripterygium wilfordii.It is used as a immune inhibitors for Clinical treatment ofdiseases of the immune system. Recently it is reported in the literatures that Triptolidecan inhibit proliferation and induce apoptosis of glioma cell．However,the effects oftriptolide on glioma invasion remain unknown．In present study,we were to detect the effects of triptolide on proliferation andinvasion in human glioma U87cells. Furthermore，we were to probe the possiblemechanism involved in triptolide induced invasive activities，and to initially study theNF-κB pathway and triptolide glioma role relationship in this event，providing thebasis for developing triptolide into a high-effective anti-glioma drug．Methods:1MTT assay was used to determine the growth inhibition by triptolide in glioma U87cells；2Tumor invasion and migration were examined by Transwell Chamber assayand Wound-healing method；3Real-Time PCR was conducted to detect including the alteration ofCathepsins B(CB) and matrix metalloproteinase9(MMP-9).4The alteration of p65expression were studied by Westerm b1ot.Results:1．Triptolide could significative inhibite glioma U87cell proliferation in adose-effect manner, IC50is25.36μg·L-1;2． Triptolide could reduce the cell ability of migration and Invasion.Wound-healing method result showed after72h cultivation,the migration of controlgroup cell were45±3.Cell morphological and growth was great.while Triptolidegroup were11±2,. Cellular pseudopod become shorter, and vitality decline(P<0.05).3．RealTime PCR:Compared with control group，the mRNA of CB and MMP-9gene expressions level of Triptolide group decreased after24h. Show CB and MMP-9transcription significantly was suppressed. the2-△△CTvalues of CB gene was0.64and MMP-9gene was0.28(all P<0.05).4．Western Blot:Through the Western Blot detection Triptolide down-regulatingp65active after24h. At the same time Triptolide mainly block the protein level ofMMP-9and CB.Conclusions:1.In vitro, triptolide can repressing cell proliferation and invasion in humanglioma．2.Triptolide could depress the invasion of glioma cells owing to down-regulatingthe level of NF-κB pathway which could inhibit CB and MMP9activities,reduce theability of hydrolyzing extracellular matrix in vitro．... |
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